Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(1): 667-675
DOI: https://doi.org/10.20546/ijcmas.2020.901.072


In Vitro Growth, Antrum Formation and Metaphase II Development of Preantral Follicles in Sheep with Estradiol-17β Supplementation at Different Time Points
S. S. Varaprasad Reddy1, B. R. Naik1, A. V. N. Sivakumar1*, Punyakumari2 and J. Suresh3
1Dept. of Veterinary Physiology, 2Dept. of AGB, 3CCVEC, Sri Venkateswara Veterinary University, Tirupati - 517502, Andhra Pradesh, India
*Corresponding author
Abstract:

The present study was conducted to investigate the influence of sequential addition of estradiol-17β on in vitro development of preantral follicles (PFs’) in sheep. Preantral follicles isolated from the ovarian cortical slices using micro dissection method were cultured for six days in Bicarbonate buffered Tissue culture medium 199B (TCM 199B) or in a standard culture medium supplemented with Estradiol-17β (5 ng/ml) at different points during the culture period. COCs isolated from the follicles at the end of six day culture in different treatments were subjected to in vitro maturation for additional 24h. Supplementation of estradiol-17β during first two days of the culture supported better average increase in diameter and proportion of PFs' exhibiting antrum formation at the end of six day culture. Further the oocytes in COCs isolated at the end of culture in these treatments and subsequently subjected to in vitro maturation (IVM) for 24hr developed at a higher frequency to MII (metaphase II) stage. Supplementation of Estradiol-17β to TCM 199B culture medium in early stages followed by standard medium alone in later stages supports better development of PFs' in vitro. Following supplementation with estradiol-17β for the first two days culture of PFs' in standard medium appears to be advantageous for the development of preantral follicles in vitro.


Keywords: In vitro, Preantral follicles, Estradiol-17β, Sheep
Download this article as Download

How to cite this article:

Varaprasad Reddy. L.S.S, B. R. Naik, A. V. N. Sivakumar, B. Punyakumari and Suresh. J. 2020. In vitro Growth, Antrum Formation and Metaphase II Development of Preantral Follicles in Sheep with Estradiol-17β Supplementation at Different Time Points.Int.J.Curr.Microbiol.App.Sci. 9(1): 667-675. doi: https://doi.org/10.20546/ijcmas.2020.901.072