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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(1): 556-564
DOI: https://doi.org/10.20546/ijcmas.2020.901.061


Evaluation of Nested PCR in Blood, Urine and Stool in Detection of Typhoid Fever by Sequences in the VI Region of the Flagellin Gene
Aalaa Abouelnour1*, Maysaa El Sayed Zaki1, Sherif M. H. Elkannishy2, 3*
1Department of Clinical Pathology, Faculty of Medicine, Mansoura-35516, Egypt
2Department of Toxicology, Mansoura Hospital, Mansoura University,
Mansoura-35516, Egypt
3Department of Pharmacology and Toxicology, Faculty of Pharmacy, University of Tabuk, Tabuk, 71491, Saudi Arabia
*Corresponding author
Abstract:

Typhoid fever remain a leading cause of morbidity, mortality and economic loss regardless of age or gender in developing countries. A sensitive and specific test is urgently needed for the accuracy of the diagnosis. The clinical pictures is commonly misleading with other ferial diseases and diagnosis depends mainly on the laboratory. By using molecular method especially nested PCR it provides the highest sensitivity and specificity. To evaluate the sensitivity and specificity nested polymerase chain reaction (PCR) specific for Salmonella enterica serovar Typhi was used for the detection of the pathogen in blood, urine, and stool samples. This was a prospective study which involved from 81patients with clinical suspicion of typhoid fever. In addition to 20 patients with febrile condition of cause other than tyhoid. For each patient blood culture and widal test was done as in the routine lab processes of febrile condition. Out of 81 suspected cases of typhoid fever, nested PCR was able to detect gene sequences specific for S. typhi in 76 cases in blood and 56 cases in urine specimens each. and 39 cases in stool specimens However, S. typhi was isolated from only 32 cases in blood culture None of the febrile controls tested positive in blood culture, or PCR.


Keywords: Typhoid fever –nested Pcr -flagellin gene
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How to cite this article:

Aalaa Abouelnour, Maysaa El Sayed Zaki, Sherif M. H. Elkannishy. 2020. Evaluation of Nested PCR in Blood, Urine and Stool in Detection of Typhoid Fever by Sequences in the VI Region of the Flagellin Gene.Int.J.Curr.Microbiol.App.Sci. 9(1): 556-564. doi: https://doi.org/10.20546/ijcmas.2020.901.061