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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(1): 37-45
DOI: https://doi.org/10.20546/ijcmas.2020.901.005


Evaluation of Microbial Contamination using various Air Sampling Techniques in Operation Theatres
Vijitha Alva and Thomas S. Kuruvilla*
Department of Microbiology, Father Muller Medical College, Mangalore, Karnataka- 575002, India
*Corresponding author
Abstract:

Microbial contamination of the Operation theatres (OT’s) continues to increase the prevalence of nosocomial infections. Our study evaluates the microbial contamination using various air sampling techniques in OT’s and find a suitable technique to assess air quality. A total 151 air samples were collected from various OT’s of our tertiary care centre.76 samples were collected by an active method using an air slit sampler and 75 samples were collected by passive settle plate method using 5% Sheep blood agar in 9cm petri-plates. The mean microbial count of the both active and passive sampling method in OT’s was 16.439 colony forming units (CFU)/m3 and 13.480 CFU/m3 respectively. In most OT’s CFU/m3 were in normal limits. The labor OT at rest, exceeded the maximum limit value of active method <35 CFU/m3 and of the passive method <5CFU/9cm/h. The Oncology OT also exceeded the maximum limit value of the active method <180CFU/m3 and passive method <25 CFU/9cm/h. The most common isolate in both methods was Coagulase negative Staphylococcus (CoNS). The present study demonstrates that the active sampling method is better than the passive method to judge the microbial air quality in our tertiary care centre and the most common isolate was CoNS.


Keywords: Air sampler, Operation theaters, Air contamination, mean microbial count
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How to cite this article:

Vijitha Alva, K. and Thomas S. Kuruvilla. 2020. Evaluation of Microbial Contamination using various Air Sampling Techniques in Operation Theatres.Int.J.Curr.Microbiol.App.Sci. 9(1): 37-45. doi: https://doi.org/10.20546/ijcmas.2020.901.005