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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(12): 3012-3019
DOI: https://doi.org/10.20546/ijcmas.2019.812.350


Bacteriological Profile and Antibiotic Susceptibility Pattern of Isolates from Ascitic Fluid among Patients Diagnosed With Chronic Liver Disease in a Tertiary Care Hospital: An Observational Study
B. M. Manasa*, Savitha Nagaraj and Harshad Devarbhavi
Department of Microbiology, Subbaiah Institute of Medical Sciences, Shivamogga, India
*Corresponding author
Abstract:

Spontaneous bacterial peritonitis (SBP) is a condition where there is development of monomicrobial infection of the ascitic fluid in the absence of any intra-abdominal source of infection and raise in the polymorphonuclear leukocyte cells (PMN) more than 250cells/mm3. To describe the bacteriological profile and antibiotic susceptibility pattern of isolates in ascitic fluid from patients with SBP. Records of 500 patients with suspected diagnosis of SBP were analyzed for two years (January 2015- December 2016). All patients had undergone paracentesis after admission to the hospital. Ascitic fluid was cultured by automated blood culture (BacT/Alert) method. Antibiotic sensitivity testing was carried out by Kirby- Bauer disc diffusion method. Out of 500 cases of clinically suspected SBP, 483 (96.6%) were males and 12 (3.4%) were females. Study showed 25.6% (n=128) of subjects had SBP. Classical SBP was seen in 28 (21.8%), Bacterascites in 54 (42.1%) and Culture negative neutrocytic ascites (CNNA) in 46 (35.93%). Escherichia coli was isolated from 45(48.9%) cases, Acinetobacter spp. from 8 (8.88%), Pseudomonas spp. from 2 (2.22%), Klebsiella spp. was isolated from 7 (7.77%), Enterobacter species from 1(1.%), Enterococcus spp. from 17 (18.47%) and Staphylococcus aureus from 2(2.22%). Resistance to cephalosporins and quinolones 45.5% and 28.9% were observed respectively. Escherichia coli and Enterococcus spp. were the most common organisms isolated. More than 50% of the Gram negative bacteria are resistant to the cephalosporins which are the most frequently used antibiotic.


Keywords: Bacteriological profile, Ascitic fluid, Chronic liver disease, Antibiotic susceptibility
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How to cite this article:

Manasa B. M., Savitha Nagaraj and Harshad Devarbhavi. 2019. Bacteriological Profile and Antibiotic Susceptibility Pattern of Isolates from Ascitic Fluid among Patients Diagnosed With Chronic Liver Disease in a Tertiary Care Hospital: An Observational Study.Int.J.Curr.Microbiol.App.Sci. 8(12): 3012-3019. doi: https://doi.org/10.20546/ijcmas.2019.812.350