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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(12): 2647-2653
DOI: https://doi.org/10.20546/ijcmas.2019.812.309


Evaluation and Validation of Rice (Oryza sativa L.) Genotypes for Tissue Culture Response from Mature Embryos
Aafreen Sakina1, Zahoor A. Rather2, Saba Mir1, Sajad M. Zargar1, Imtiyaz Murtaza3, Nagina Nazir4, Ambreena Din2 and Asif B. Shikari1*
1Division of Plant Biotechnology, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir (SKUAST-K), Shalimar, J&K, 190025, India
2Division of Floriculture & Landscaping, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir (SKUAST-K), Shalimar, J&K, 190025, India
3Division of Basic Sciences and Humanities, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir (SKUAST-K), Shalimar, J&K, 190025, India
4Division of Agricultural Statistics, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir (SKUAST-K), Shalimar, J&K, 190025, India
*Corresponding author
Abstract:

The biotechnological intervention to enhance crop productivity via genetic transformation mainly depends upon an efficient regeneration protocol. Japonica, Indica and Basmati varieties of rice vary highly in their regeneration capabilities. Establishment of a highly efficient regeneration system for mature embryos in rice will enable transformation of foreign genes in rice. In this study, 12 genotypes of rice representing japonica, indica and basmati varieties were assessed for their calli and green plant regeneration frequencies. The mature seeds of the genotypes were inoculated on MS media supplemented with auxin (2,4-D @ 2.5 mg/l), cytokinin (kinetin@ 0.5mg/l) and 3% sucrose. They exhibited variable calli induction and green plant regeneration frequencies. Calli regeneration frequency ranged from 100% - 20%, highest being in GS-88, Kamad and PS-3 (100%) and lowest in Kohsar (20%). Highest regeneration frequency was obtained in K-332 and Kamad. Among all the varieties K-332, GS-88 and Kamad performed well in respect of callus induction and green plant regeneration. The plants were successfully acclimatized and transferred to pots.


Keywords: Rice, Tissue culture embryo, Genotypes, Regeneration
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How to cite this article:

Aafreen Sakina, Zahoor A. Rather, Saba Mir, Sajad M. Zargar, Imtiyaz Murtaza, Nagina Nazir, Ambreena Din and Asif B. Shikari. 2019. Evaluation and Validation of Rice (Oryza sativa L.) Genotypes for Tissue Culture Response from Mature Embryos.Int.J.Curr.Microbiol.App.Sci. 8(12): 2647-2653. doi: https://doi.org/10.20546/ijcmas.2019.812.309