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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(12): 2641-2646
DOI: https://doi.org/10.20546/ijcmas.2019.812.308


Marker Based Screening of F1 (Firdous x Gala) Mapping Population for Major Scab Resistance Gene Rvi6 in Apple (Malus × Domestica)
Saba Mir1, Khalid Z. Masoodi1, Aafreen Sakina1, Khalid M. Bhat2, Bilal A. Padder3, Imtiyaz Murtaza4, Nagina Nazir5 and Asif B. Shikari1*
1Division of Plant Biotechnology, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir (SKUAST-K), Shalimar, J&K-190025, India
2Division of Fruit Science, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir (SKUAST-K), Shalimar, J&K-190025, India
3Division of Plant Pathology, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir (SKUAST-K), Shalimar, J&K-190025, India
4Division of Basic Sciences and Humanities, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir (SKUAST-K), Shalimar, J&K-190025, India
5Division of Agricultural Statistics, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir (SKUAST-K), Shalimar, J&K-190025, India
*Corresponding author
Abstract:

Scab caused by Venturia inaequalis (Cke.) Wint. is the most important fungal disease of apple. The development and deployment of genetic resistance is reliable and a safe option to combat the dreaded disease with palpable advantage as compared to chemical control. A variety Firdous developed by SKUAST-K has been known for its resistance, however, genetic basis of resistance has not been proved or established till date. An initiative was taken to cross the scab resistant variety, Firdous to susceptible cultivar Gala that resulted into population of 110F1 plants. Among these derived F1s 46 were tested for presence of Vf gene using the linked SCAR marker. The marker analysis helped us to identify resistance specific alleles for gene Rvi6 in 25 out of 46 F1s. The F1 population was also screened using a mono-conidial mixture of four races viz., (0), (1), (2) and (1, 2) under controlled conditions. A fair degree of correlation existed between allelic information and reaction phenotype that indicated the presence of Rvi6 in Firdous. Further, the F1 population was screened for the markers linked to fruit firmness. The combined information of both the markers may help us to identify individuals which are resistant to scab and carry favourable alleles for fruit firmness required for better storage and quality.


Keywords: Apple, Scab, Markers, Resistance, Gene, Rvi6
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How to cite this article:

Saba Mir, Khalid Z. Masoodi, Aafreen Sakina, Khalid M. Bhat, Bilal A. Padder, Imtiyaz Murtaza, Nagina Nazir and Asif B. Shikari. 2019. Marker Based Screening of F1 (Firdous x Gala) Mapping Population for Major Scab Resistance Gene Rvi6 in Apple (Malus × Domestica).Int.J.Curr.Microbiol.App.Sci. 8(12): 2641-2646. doi: https://doi.org/10.20546/ijcmas.2019.812.308