National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Bioremediation, which employs the biosorption and or biodegradation potentials of organisms or their attributes, is an effective technology that can be used to accomplish both effective detoxification and volume reduction. Research was carried out to further provide experimental evidences that support the use of biological method which is a better, less expensive and safer means of biosorbing hydrocarbon contaminants. Bacteria isolated from the African locust bean (Parkia biglobosa) effluent namely; Bacillus cereus, B. subtilis, Pseudomonas aeruginosa and Acetobacter spp were used to biodegrade crude-oil products such as petrol, kerosene and diesel oil at varying concentrations in a culture medium. The pattern of microbial growth differs from organism to organism due to several factors such as the incubation period, the nature and composition of the nutrient in which the organism was cultured. Microbial load was between the range of 4.0-5.0 x 106 Cfu/ml for B. cereus, B. subtilis, P. aeruginosa and Acetobacter spp when cultured in 0.2-0.4 ml petrol concentration. Also, increase in the concentration of the hydrocarbon in the medium from 0.2ml to 0.6ml led to a declination of cell density in all the four microbes investigated. This work established the fact that B. cereus proved to be a better hydrocarbon degrader as compared to the other bacteria isolates used in this study in this order: B. cereus > B. subtilis > P. aeruginosa > Acetobacter spp. In conclusion, this work revealed that Bacillus cereus, B. subtilis, Pseudomonas aeruginosa and Acetobacter species isolated from effluent collected from production site of fermented African locust beans condiment are able to utilize hydrocarbon compounds as their carbon source, thereby proving their biodegradability potentials.
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