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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(12): 1430-1438
DOI: https://doi.org/10.20546/ijcmas.2019.812.173


Study on Melanized Shrimp Reveals Bacillus sp and Acinetobacter sp as Potential Sources for Bacterial Tyrosinase
Muthulakshmi*1, U. Sivakumar2, Ranjit Kumar Nadella1, V. Murugadas1, S.S. Greeshma1 and M.M. Prasad1
1MFB Division, ICAR-Central Institute of Fisheries Technology, Willingdon Island, Matsyapuri P.O., Cochin, Kerala, India 682029
2Department of Microbiology, Tamil Nadu Agricultural University,
Coimbatore, 641003, India
*Corresponding author
Abstract:

Melanin is a biopolymer produced by phenol oxidases group of enzymes and plays an important role in radiational, thermal and biochemical stress tolerance. This pharmacologically active pigment has been reported to be produced by bacteria. However, there is a paucity of data for bacteria associated with melanisation in shrimp. In this context the present study was undertaken.  The presence of bacteria with the extracellular phenol oxidase production from the melanized appendages of Penaeus vannamei is studied. Market drawn samples of white leg shrimp with melanization were checked for possible intervention of bacteria with phenol oxidase production. Out of 24 isolated checked, three bacterial isolates viz, TMA7, TMA9 and TMA10 shown potential phenol oxidase production by positive and negative screening with tyrosine. The sequencing analysis of 16S rRNA fragment confirmed that the TMA7, TMA9, TMA10 bacterial isolates were Bacillus sp, Acinetobacter sp, and Bacillus megaterium respectively. The study also suggests that Bacillus sp and Acinetobacter sp could be a potential source for tyrosinase which can be used in the fish processing industry.


Keywords: Tyrosinase, Phenol oxidase, Melanization, Bacillus sp
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How to cite this article:

Muthulakshmi, T., U. Sivakumar, Ranjit Kumar Nadella, V. Murugadas, S.S. Greeshma and Prasad, M.M. 2019. Study on Melanized Shrimp Reveals Bacillus sp and Acinetobacter sp as Potential Sources for Bacterial Tyrosinase.Int.J.Curr.Microbiol.App.Sci. 8(12): 1430-1438. doi: https://doi.org/10.20546/ijcmas.2019.812.173