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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(12): 1254-1260
DOI: https://doi.org/10.20546/ijcmas.2019.812.155


Nasal Carriage of Staphylococcus aureus and MRSA among Companion Animals and Livestock in Wayanad District, India
Hamna Hakim2*, Prejit1, 2, K. Asha2, Jess Vergis1, 2, P. M. Deepa3,Suma2 and Hema Persis Andrews2
1Centre for One Health Education, Advocacy, Research and Training
2Department of Veterinary Public Health, CV&AS, Pookode, India
3Department of Veterinary Preventive Medicine and Epidemiology, CV&AS, Pookode Kerala Veterinary and Animal Sciences University, Pookode, Wayanad-673576, India
*Corresponding author
Abstract:

Antimicrobial resistance (AMR) is one of the leading threats in healthcare management system. Staphylococcus aureus is a commensal bacteria commonly seen on skin and nasal passage that can turn as an opportunistic pathogen. The present study was carried out to evaluate the nasal carriage of S. aureus and methicillin-resistant S. aureus (MRSA) among apparently healthy companion animals and livestock. A total of 160 nasal swabs were collected from dogs (30), cats (30), cattle (40), goats (30), and pigs (30). All the samples were initially screened for prevalence of S. aureus, which was confirmed by PCR using species specific primer (nuc gene). All the S. aureus isolates were screened for occurrence of MRSA, both phenotypically (by double disc diffusion using cefoxitin and oxacillin discs) and genotypically (by PCR targeting mecA gene). There was significant difference in nasal carriage of S. aureus among animal population (Chi-square value – 33.206, p-value < 0.001), which was found as 76.67 per cent, 23.33 per cent, 53.33 per cent, 62.5 per cent, and 86.67 per cent among dogs, cats, cattle, goats, and pigs respectively. None of the nasal swabs from animals were positive for MRSA. The present study signifies the occurrence of nasal carriage of S. aureus and absence of MRSA among domestic animal population of Wayanad district.


Keywords: Cefoxitin, Oxacillin, Dogs (30), Cats (30), Cattle (40), Goats (30), Multi-drug resistance
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How to cite this article:

Hamna Hakim, Prejit, K. Asha, Jess Vergis, P. M. Deepa, N. Suma and Hema Persis Andrews. 2019. Nasal Carriage of Staphylococcus aureus and MRSA among Companion Animals and Livestock in Wayanad District.Int.J.Curr.Microbiol.App.Sci. 8(12): 1254-1260. doi: https://doi.org/10.20546/ijcmas.2019.812.155