Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2019) [Effective from January 1, 2019]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(12): 2522-2526
DOI: https://doi.org/10.20546/ijcmas.2019.812.295


Molecular Diagnosis of Babesia canis Infection with Concurrent of Evans Syndrome in a Labrador Dog
M. Saravanan*, P. Selvaraj, S. Yogeshpriya, M. Venkatesan, P.K. Ramkumar and K. Kannan
Veterinary Clinical Complex, Veterinary College and Research Institute, Orathanadu, Thanjavur – 614 625, TamilNadu Veterinary and Animal Sciences University (TANUVAS), India
*Corresponding author
Abstract:

Clinical case is five years old female Labrador dog presented to Small Animal Medicine unit of TVCC, VCRI, Orathanadu. History is dullness and anorexia two days. Recurrent tick infestations, anemic and thrombocytopenic crisis were regularly. Vaccination and deworming were regularly. Physical examination revealed dullness, pale mucosa, vital parameters were normal and ticks on interdigital space and in ears were noticed. Distended abdomen, hepatosplenomegaly and petechial hemorrhage on ventral abdomen were observed. Hematology revealed anemia and thrombocytopenia. Negative for blood born parasites in peripheral blood smear. Serum biochemical and urine analysis found to be normal. Serum and EDTA blood were submitted for molecular testing. Flow cytometry revealed Immune-mediated hemolytic anemia (IMHA) and Immune-mediated thrombocytopenia (IMTP). MAT negative for leptospira. Babesia canis positive by PCR method at 394bp. Haematology alteration was studied before and after therapy. Ultrasonography revealed hepatosplenomegaly. Based on PCR and flow cytometry the case was confirmed Evans syndrome with Concurrent Babesia canis in a Labrador dog. Dog treated with Inj. Diminazene aceturate @ 3.5mg/kg IM single dose, Inj. Ringers lactate @ 100 ml IV, Inj. Ascorbic acid @ 5 ml IV, Inj. Vitamin B complex @ 1ml IV, Inj. Prednisone @ 0.5mg/ kg IM, Tab. Doxycycline @ 10mg/kg PO and Syrup. Thrombup @ 15 ml BID PO. Dog showed clinical improvement as well as hematology parameters.


Keywords: Babesia canis, IMTP, IMHA, Flow cytometry, Evans syndrome
Download this article as Download

How to cite this article:

Saravanan, M., P. Selvaraj, S. Yogeshpriya, M. Venkatesan, P.K. Ramkumar and Kannan, K. 2019. Molecular Diagnosis of Babesia canis Infection with Concurrent of Evans Syndrome in a Labrador Dog.Int.J.Curr.Microbiol.App.Sci. 8(12): 2522-2526. doi: https://doi.org/10.20546/ijcmas.2019.812.295