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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(12): 2412-2418
DOI: https://doi.org/10.20546/ijcmas.2019.812.283


Development of Liquid Nitrogen Free and Efficient DNA Isolation Method for Plants Rich in Volatile Oil, Secondary Metabolites and Polysaccharides
Manish Kumar Suthar*, V. Thondaiman, Parmeshwar Lal Saran and Manish Kumar Mittal
ICAR-Directorate of Medicinal and Aromatic Plants Research, Anand, Gujarat-387310, India
*Corresponding author
Abstract:

Ocimum sanctum L. or “Tulsi” or Holy basil is a plant well known for its medicinal properties. This plant is rich in essential oil sequestered in peltate glands. The Tulsi is rich source of eugenol, methyl eugenol, chavicol, ursolic acid, rosmerinic acid and other phenolics and terpenes. Aloe barbadensis and Plantago ovata are plants rich in polysaccharides. These plants are very important medicinal plants. A. barbadensis is source of aloin which is used in various cosmetic products and P. ovata is the main source of Isabgol-Husk. DNA based studies in these and similar plants require optimization of DNA isolation method due to presence of high level of secondary metabolites and polysaccharides. Which generally hamper all downstream enzymatic analysis like PCR amplification. DNA isolated using standard DNA isolation method especially from stored leaves from such plants is not suitable for downstream enzymatic analysis like PCR. In present study, a DNA isolation method has been developed which results in high quality and intact DNA from Tulsi, A. barbadensis and P. ovata. This method is free from the use of liquid nitrogen, Phenol, and Cetyltrimethyl ammonium bromide and moreover efficient to isolate intact DNA which can be easily used for downstream molecular analysis. DNA isolated by newly developed method was successfully amplified by PCR reaction using random and specific primers.


Keywords: Medicinal plants, volatile oil, Polysaccharides, DNA isolation, Secondary metabolites
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How to cite this article:

Manish Kumar Suthar, V. Thondaiman, Parmeshwar Lal Saran and Manish Kumar Mittal. 2019. Development of Liquid Nitrogen Free and Efficient DNA Isolation Method for Plants Rich in Volatile Oil, Secondary Metabolites and Polysaccharides.Int.J.Curr.Microbiol.App.Sci. 8(12): 2412-2418. doi: https://doi.org/10.20546/ijcmas.2019.812.283