Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(12): 2368-2375
DOI: https://doi.org/10.20546/ijcmas.2019.812.279


In vitro Studies on Plant Regeneration in Elite Clones of Cocoa (Theobroma cacao L.)
S. Sreelekshmi* and B. Suma
Department of Horticulture, KAU P.O., Kerala Agricultural University, Thrissur-680656, Kerala, India
*Corresponding author
Abstract:

An experiment was carried out at Department of Plantation Crops and Spices, College of Horticulture, Vellanikkara with an objective to develop a protocol for in vitro regeneration and also to determine the variation in morphogenesis and regeneration among elite cocoa clones. The experiment was done in two factorial CRD using five cocoa varieties, CCRP 2, CCRP 6, CCRP 8, CCRP 15 and Scavina. Single nodded cuttings of 2-3 cm collected from the budded plants maintained in polyhouse were used as explants. The best surface sterilization of explants was achieved using Mancozeb 0.2 per cent, Streptocycline 0.1 per cent, Ethyl Alcohol 70 per cent and Mercuric Chloride 0.1 per cent. Axillary bud sprouting was induced in basal Woody Plant Media (WPM) fortified with 2-Isopentinyl Adenine (2ip) and Indole-3-Acetic Acid (IAA). The period of single shoot induction from leaf axils was lowest in variety CCRP 15 in media WPM+2ip 1ppm+IAA 0.02ppm. Among the varieties, highest multiple shoot induction percentage was recorded in variety Scavina.


Keywords: In vitro, Cocoa, explants, Woody Plant Media, 2-Isopentinyl Adenine, Indole-3-Acetic Acid
Download this article as Download

How to cite this article:

Sreelekshmi, S. and Suma, B. 2019. In vitro Studies on Plant Regeneration in Elite Clones of Cocoa (Theobroma cacao L.).Int.J.Curr.Microbiol.App.Sci. 8(12): 2368-2375. doi: https://doi.org/10.20546/ijcmas.2019.812.279