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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(12): 2263-2276
DOI: https://doi.org/10.20546/ijcmas.2019.812.269


Prevalence of Giardia intestinalis and Cryptosporidium parvum Parasites in Drinking Water in Menoufia Governorate, Egypt
Dalia Shafey1, Mohammed M. Aboamer2, Karema Abd Elhady Diab3, Heba Mohamed Abdallah4, Marwa F. Yousef 5 and Marwa Ahmed Gouda1*
1Department of Clinical and Molecular parasitology, National Liver Institute (NLI), Menoufia University, Shebin Al Koom, Menoufia, Egypt
2Manager of Biology Lab at Tala Holding Company of Water,
3Department of Clinical Pathology,
4Department of Epidemiology and Preventive Medicine, National Liver Institute(NLI), Menoufia University, Shebin Al Koom, Menoufia, Egypt
*Corresponding author
Abstract:

Entero-parasites protozoan of the genera Cryptosporidium and Giardia have emerged over the past decades as major waterborne pathogens with an increasing number of outbreaks reaching over one hundred and sixty. In line with the national goals of providing safe drinking water supplies, this research was designed. We aimed to estimate the prevalence of Cryptosporidium parvum and Giardia intestinalis infections in surface water plants from ShebinAl-Kom and Tala, Menoufia Governorate and to evaluate routine water purification methods used for parasitic elimination. The following methods were used for assessment of samples; mechanical filtration, staining techniques and qPCR assay for detecting DNA of (oo) cysts n water samples.Water samples collected over one year (four seasons) were 87 samples. Microscopic examination (our golden test) revealed, 49 samples positive for cryptosporidium, fourteen positive samples for Giardia and four samples were positive for both of them whereas, qPCR revealed only eight positive samples for Giardia and ten positive samples for cryptosporidium. A statistically significant result concerning seasonal variation was documented where water contamination crested in Summer (p < 0.05). The intensity of infection decreased significantly after water treatment in both Shebin and Tala water stations (P= 0.01).From the present survey, we found that the prevalence of giardiasis and cryptosporidiosis accounted for 29.8% and 47.1% in Shebin Al-Kom and Tala respectively. The parasitic cyst wall structure rendered diagnosis by qPCR. Genotyping of water samples is recommended for sourcing of infection which may be accused of water-borne outbreaks.


Keywords: Cryptosporidium, Giardia, Prevalence, Drinking water, Egypt
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How to cite this article:

Dalia Shafey, Mohammed M. Aboamer, Karema Abd Elhady Diab, Heba Mohamed Abdallah, Marwa F. Yousef and Marwa Ahmed Gouda. 2019. Prevalence of Giardia intestinalis and Cryptosporidium parvum Parasites in Drinking Water in Menoufia Governorate, Egypt.Int.J.Curr.Microbiol.App.Sci. 8(12): 2263-2276. doi: https://doi.org/10.20546/ijcmas.2019.812.269