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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(2): 1551-1558
DOI: http://dx.doi.org/10.20546/ijcmas.2017.602.173


Comparative Study on Serodiagnosis of Bovine Leptospirosis by Microagglutination Test (MAT) and Indirect ELISA
Blessy Rajan, Sumanth Kumar, Raghavan Madhusoodanan Pillai, Prabhakar Xavier Antony, Hirak Kumar Mukhopadhyay, Sangeetha Balakrishnan, Nobal Rajkumar and Mouttou Vivek Srinivas*
Department of Veterinary Microbiology, Rajiv Gandhi Institute of Veterinary Education and Research, Pondicherry-605 009, India
*Corresponding author
Abstract:

The present study was undertaken to identify the presence of anti-leptospiral antibodies in the bovine serum samples using two serological tests, a genus specific indirect ELISA and a serovar specific microagglutination test (MAT). A total of 250 serum samples from bovine leptospirosis suspected cases were sampled in and around Pondicherry (Southern India). The anit-leptospiral antibodies were detected by indirect ELISA using outer membrane proteins (OMP’s) of Leptospira interrogans serovar pomona as antigen and compared with MAT using six infecting serovars (hardjo, icterohaemorrhagiae, grippotyphosa, pomona, canicola and australis) as antigen. Statistical analysis of the data was performed using chi-square test. Out of 250 sera samples, 91 (36.4 %) and 62 (24.8%) were positive by indirect ELISA and MAT respectively. The indirect ELISA employed in this study revealed a very high sensitivity of 98.38 % in comparison to 67.03 % in MAT. Chi –square analysis revealed a significant difference (i.e. P<0.001) between the two serological tests. This study revealed that indirect ELISA was found to be highly sensitive, rapid and easy to perform for detection of bovine anti-leptospiral antibodies in comparison with MAT.


Keywords: Bovine leptospirosis, Indirect ELISA, Outer membrane protein (OMP), Microagglutination test (MAT).
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How to cite this article:

Blessy Rajan, Sumanth Kumar, Raghavan Madhusoodanan Pillai, Prabhakar Xavier Antony, Hirak Kumar Mukhopadhyay, Sangeetha Balakrishnan, Nobal Rajkumar and Mouttou Vivek Srinivas. 2017. Comparative Study on Serodiagnosis of Bovine Leptospirosis by Microagglutination Test (MAT) and Indirect ELISA.Int.J.Curr.Microbiol.App.Sci. 6(2): 1551-1558. doi: http://dx.doi.org/10.20546/ijcmas.2017.602.173