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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(2): 1534-1539
DOI: http://dx.doi.org/10.20546/ijcmas.2017.602.171


Flow Cytometric Analysis of T cell Subset in Bursa of Fabricius in Broiler Chicken (Gallus domesticus)
Jayachandra Kempashi1, T.A. Kannan2*, Sabiha Hayath Basha1, A.Raja3 and Geetha Ramesh1
1Department of Veterinary Anatomy, Tamil Nadu Veterinary and Animal Sciences University, Madras Veterinary College, Chennai- 600 007, India
2Centre for Stem Cell Research and Regenerative Medicine, Tamil Nadu Veterinary and Animal Sciences University, Madras Veterinary College, Chennai- 600 007, India
3Department of Animal biotechnology, Tamil Nadu Veterinary and Animal Sciences University, Madras Veterinary College, Chennai- 600 007, India
*Corresponding author
Abstract:

Bursa of Fabricius, the primary lymphoid organ for B-lymphopoiesis in Avian species and is responsible for humoral immunity. Flow cytometric analysis of Bu-1a+ B-cell count and CD3+, CD4+ and CD8+ T-cell count were conducted in bursa of Fabricius of four different age groups of Nandanam broiler chicken viz., day-old, two, four and six weeks. Twelve birds from each age group, from both vaccinated and non-vaccinated groups were utilised. Tissue samples of the bursa were collected aseptically and processed for flow cytometric studies using monoclonal antibodies. Mean Bu-1a+, CD3+ and CD4+ cell count was highest in four week-old birds and lowest in day-old birds, whereas CD8+ cell count was highest in four week-old birds and lowest in six week-old birds in non-vaccinated group. In vaccinated group, Bu-1a+ cell count was highest in two week-old and lowest in six week-old birds. The mean CD3+ and CD4+ cell count was highest in four week-old birds and lowest in two week-old birds, whereas CD8+ cell count was highest in four week-old and lowest in six week-old birds in vaccinated group.


Keywords: Bursa of fabricius, T cell subset, Nandanam chicken, Flowcytometric analysis.
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How to cite this article:

Jayachandra Kempashi, T.A. Kannan, Sabiha Hayath Basha, A. Raja and Geetha Ramesh. 2017. Flow Cytometric Analysis of T cell Subset in Bursa of Fabricius in Broiler Chicken (Gallus domesticus)Int.J.Curr.Microbiol.App.Sci. 6(2): 1534-1539. doi: http://dx.doi.org/10.20546/ijcmas.2017.602.171