National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
New duck disease/ riemerellosis, caused by Riemerella anatipestifer, is a predominant disease affecting ducks, causing severe economic loss to the farmers of Kerala. However, the disease has also been reported from other birds. Ducks of all age groups are susceptible, but high mortality was observed in ducklings compared to adults. Vaccination is the only strategy for the control of the disease. As a preliminary approach for the development of vaccine, determination of LD50 for R. anatipestifer in host system is required and the present study is envisaged for the same. Study groups of birds were inoculated with different concentrations of the bacteria via subcutaneous route. Mortality was recorded two weeks post- inoculation. All the dead birds were examined for specific gross lesions of riemerellosis and re-isolation of the organism was attempted on blood agar from tissues containing lesions. Concentration of 2.5 OD values at 525 nm with a dose of 1 mL per bird subcutaneously was selected as LD50.
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