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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(12): 680-684
DOI: https://doi.org/10.20546/ijcmas.2019.812.089


Determination of Lethal Dose (LD50) of Riemerella anatipestifer
Rinsha Balan1, P. M. Priya1*, M. Mini1, Binu K. Mani2, Vrinda Menon3 and P. Anitha4
1Department of Veterinary Microbiology, 3Department of Veterinary Public Health,
4Department of Poultry Science, College of Veterinary and Animal Sciences (CVAS), Mannuthy, Thrissur Dt, Kerala-680651, India
2Department of Veterinary Microbiology, College of Veterinary and Animal Sciences (CVAS), Pookode, Wayanad Dt, Kerala-673576, India
*Corresponding author
Abstract:

New duck disease/ riemerellosis, caused by Riemerella anatipestifer, is a predominant disease affecting ducks, causing severe economic loss to the farmers of Kerala. However, the disease has also been reported from other birds. Ducks of all age groups are susceptible, but high mortality was observed in ducklings compared to adults. Vaccination is the only strategy for the control of the disease. As a preliminary approach for the development of vaccine, determination of LD50 for R. anatipestifer in host system is required and the present study is envisaged for the same. Study groups of birds were inoculated with different concentrations of the bacteria via subcutaneous route. Mortality was recorded two weeks post- inoculation. All the dead birds were examined for specific gross lesions of riemerellosis and re-isolation of the organism was attempted on blood agar from tissues containing lesions. Concentration of 2.5 OD values at 525 nm with a dose of 1 mL per bird subcutaneously was selected as LD50.


Keywords: Lethal dose, LD50, Riemerella anatipestifer, New duck disease, Riemerellosis
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How to cite this article:

Rinsha Balan, P. M. Priya, M. Mini, Binu K. Mani, Vrinda Menon and Anitha, P. 2019. Determination of Lethal Dose (LD50) of Riemerella anatipestifer.Int.J.Curr.Microbiol.App.Sci. 8(12): 680-684. doi: https://doi.org/10.20546/ijcmas.2019.812.089