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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
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Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(12): 1987-1997
DOI: https://doi.org/10.20546/ijcmas.2019.812.237


Antibiotic Resistance of Arcobacter spp. Isolated from Sewage, Meat Shop and Fish Market Environment of Bareilly City
B. Harkal1*, Gulzar Ahmad1, R. S. Rathore1, S. V. S. Malik1, D. B. Rawool1
B. Dubal1, D. K. Singh1, Kumar Deepak2 and Saini Mohini3
1Department of Veterinary Public Health, 2Department of Animal Biotechnology,
3Department of Animal Biochemistry, Indian Veterinary Research Institute, Izatnagar, Bareilly-243122, Uttar Pradesh, India
*Corresponding author
Abstract:

Arcobacter genus is an emerging pathogen, with three main spp. are pathogenic (A. butzleri, A. skirrowii and A. cryaerophilus) in nature. In present study raw sewage water, chicken, mutton and fish market associated environmental samples were screened for presence of Arcobacter spp. from Bareilly city of India. Out of total 170 samples, 50 from sewage water, 40 each of meat shops (chicken, mutton, fish) associated environment such as swab samples from knife (10), chopper (10), wooden block (10) and utensils (10) from each meat shop respectively. 26/170 (15%) samples were positive for Arcobacter spp. by multiplex PCR detection and culture positive samples were 18/170 (10.58%). The 8/50 from sewage water, 8/40 from chicken shop, 4/40 mutton shop and 6/40 from fish market associated environment detected positive by m-PCR. Out of total 170 samples screened 18 were culture positive with isolation rate 6/50 (12%) from sewage water, 5/40 (12.5%) from chicken shop, 3/40 (7.5%) from mutton shop, 4/40 (10%) from fish market associated environments. Out of 18 Culture positive samples Arcobacter butzleri was predominant spp. isolated, than A. skirrowii and A. cryaerophilus and mix infection. The 18 positive strains were selected for disk diffusion, tested against 12 different types of antibiotic discs. High antibiotic resistance were seen as in cephalothin (17/18), co-trimoxazle (16/18), ampicillin (14/18), Vancomycin (14/18), tetracycline (14/18), kanamycin (12/18), Azitromycin (11/18) and other antibiotics shows greater sensitivity to Arcobacter butzleri with resistance pattern as 0/18 by nalidixic acid, Erytromycin (4/18), Ciprofloxacin (4/18), Gentamicin (5/18) and streptomycin (6/18). Multi drug resistance (MDR) was seen in this the study. Which may concerned to treatment failure in human and animal concerned to Arcobacter MDR strains. Sewage water may acts as reservoir of Arcobacter spp. as human, animal excreta are major part in and associated water. Most of the sewage water is recycled by municipal corporation. Hence water processing plant, purification methods must aware of probable public health risk related to sewage water. More cautions are recommended for personnel hygiene in processing, handling and awareness related to meat and meat products. The unnecessary use or misuse of antibiotics should be limited or avoided for public health safety.


Keywords: Sewage, Arcobacter spp., MDR strains, Public health, Meat
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How to cite this article:

Harkal, D. B., Gulzar Ahmad, R. S. Rathore, S. V. S. Malik, D. B. Rawool, Z. B. Dubal, D. K. Singh, Kumar Deepak and Saini Mohini. 2019. Antibiotic Resistance of Arcobacter spp. Isolated from Sewage, Meat Shop and Fish Market Environment of Bareilly City.Int.J.Curr.Microbiol.App.Sci. 8(12): 1987-1997. doi: https://doi.org/10.20546/ijcmas.2019.812.237