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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(12): 1704-1712
DOI: https://doi.org/10.20546/ijcmas.2019.812.205


Phytochemical Determination and Antibacterial Activity of Urtica dioica Leave Extracts against Isolated Food Borne Bacteria
Geeta Rawat1* and Alka Bameta2
1Sardar Bhagwan Singh University, Dehradun, India
2Govind Ballabh Pant University of Agriculture and Technology, Pantnagar, India
*Corresponding author
Abstract:

Aim of this study was to analyze phytochemical constituents and antibacterial activity of Urtica dioica leave extracts against isolated food-borne pathogens from different food samples. The phytochemical analysis showed the presence of carbohydrates, alkaloids, saponins, tannins and protein. Total 75 different isolates of bacteria were isolated from different food samples. Out of 75twenty-four were found to be positive for Klebsiella, twenty-six for Staphylococcus spp. and twenty-five for E. coli. Maximum zone of inhibition was recorded by ethyl acetate extract against E. coli with 2.36±0.26 mm diameter followed by Klebsiella (2.28±0.16 mm) Staphylococcus spp. (1.96±0.28 mm) respectively. Methanol extract showed maximum inhibitory zone of 1.91±0.26 mm against Klebsiella followed by 1.82±0.33 mm zone against Staphylococcus spp. and 1.5±0.17 mm inhibitory zone against E. coli. Aqueous extract not showed inhibition against any isolated bacteria.


Keywords: Antibacterial activity, Bacteria, Food-borne, Inhibitory zone, Phytochemical
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How to cite this article:

Geeta Rawat and Alka Bameta. 2019. Phytochemical Determination and Antibacterial Activity of Urtica dioica Leave Extracts against Isolated Food Borne Bacteria.Int.J.Curr.Microbiol.App.Sci. 8(12): 1704-1712. doi: https://doi.org/10.20546/ijcmas.2019.812.205