Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(12): 1685-1692
DOI: https://doi.org/10.20546/ijcmas.2019.812.202


Optimization of an efficient rapid regeneration of Indian wheat cultivars by callus induction and multiple shoot induction using mature embryos
R. Krishnaveni1,2*, D. P. Biradar1, I. S. Katageri1 and S. Shuba1
1University of Agricultural Sciences, Dharwad, India 2Vijayanagara Sri Krishnadevaraya University, Ballari-583105, Karnataka, India
*Corresponding author
Abstract:

Wheat is the second most produced staple food crop in world. An efficient method for in vitro micro propagation and genetic transformation of wheat are crucial for both basic and applied research.  The present study was initiated with a view to develop a regeneration system in 4 genotypes of wheat cultivars HD2888, HD2329, DBW148, and DBW88 as a prerequisite to transformation. The mature embryos were excised from seeds and cultured on MS medium supplemented with high and low concentrations of cytokinins and auxins respectively. Callus induction was prominent with 4mg/L 2,4D with HD2888. The regeneration system was optimised and was prominent with 1mg/L kinetin by slowly reducing the concentration of 2,4D to 2mg/L. The MS medium containing 2 mg/L N6 -benzylaminopurine (BAP) and 0.5mg/L 2,4-D was found to be effective for multiple shoot formation in HD2932 cultivar that could produce 7 shoots per explants. The elongated shoots were separated and successfully rooted on half MS medium containing 0.2 mg/L indole-3-acetic acid (IAA) Callus mediated regeneration took approximately 36 days to regenerate where as multile shoot mediated regeneration took 20 days. Further studies will be taken up to check the transformation efficiency by transforming the desired gene through Agrobacterium mediated protocols.


Keywords: Callus induction, Multiple shoot induction, Indian wheat, Mature embryos
Download this article as Download

How to cite this article:

Krishnaveni, R., D. P. Biradar, I. S. Katageri and Shuba, S. 2019. Optimization of an efficient rapid regeneration of Indian wheat cultivars by callus induction and multiple shoot induction using mature embryos.Int.J.Curr.Microbiol.App.Sci. 8(12): 1685-1692. doi: https://doi.org/10.20546/ijcmas.2019.812.202