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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(12): 1136-1151
DOI: https://doi.org/10.20546/ijcmas.2019.812.143


Antifibrotic Alleviative Effect of Bone Marrow-Derived Mesenchymal Stem Cells on Experimentally induced Schistosoma mansoni Related Liver Fibrosis
Marwa Ahmed Gouda1Ù­, Asmaa Shams El Dein Mohamed2, Naglaa Said Elabd3, Fatma Eldesoky Ahmed4, Ghada Samir Amer5 and Dalia Shafey1
1Department of Clinical and Molecular Parasitology, National Liver Institute (NLI), Menoufia University, Shebin Al Koom, Menoufia, Egypt
2Department of Pathology, 3Department of Tropical Medicine, 5Department of Physiology, Faculty of Medicine, Menoufia University, Shebin Alkoom, Menoufia, Egypt
4Department of Clinical Pharmacology, Faculty of Medicine, Menoufia University, Shebin Alkoom, Menoufia, Egypt
*Corresponding author
Abstract:

With increased national programs for eliminating schistosomiasis and viruses affecting the liver in Egypt, treating the remained pathology represented mainly by liver fibrosis will probably be a competitive goal in the coming few years. We aimed in our work to reinforce the antifibrotic effect of BM-MSCs in liver fibrosis related to Schistosoma mansoni (Egyptian strain) infection in murine model and correlating serum Hyaluronic acid (HA) to liver fibrosis. A pilot study was done first to ensure reaching of MSCs to the fibrosed liver tissues following infection with S. mansoni and secondly to detect the best route for MSCs inoculation. Then 70 female Swiss albino mice were divided into five groups. GI; uninfected untreated, (NC, n=10), infected mice were categorized into 4 groups each contained 15 mice regarding treatment at 6th week p.i; GII: Infected untreated, GIII: PZQ treated, GIV: received MSCs and GV: PZQ then MSCs treated. Sacrifaction of all mice at 14th week p.i. was done and mice were subjected to parasitological, biochemical, histological and immunohistochemical workup. We observed that the use of BM-MSCs and PZQ significantly decreased mean granuloma number, with significant reduction in fibrosis percent (Masson trichrome) staining than PZQ. Presence of fibrosis was associated with high serum HA level and reduction of fibrosis was linked to lowering its serum levels. Use of the combined treatment improved liver function tests. Intravenous route for administration of MSCs and the use of HA as a noninvasive marker of fibrosis are recommended. MSCs significantly alleviate liver fibrosis induced by Schistosoma mansoni infection.


Keywords: BM-MSCs, Schistosoma mansoni, Praziquantel, Hyaluronic acid, α SMA
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How to cite this article:

Marwa Ahmed Gouda, Asmaa Shams El Dein Mohamed, Naglaa Said Elabd, Fatma Eldesoky Ahmed, Ghada Samir Amer and Dalia Shafey. 2019. Antifibrotic Alleviative Effect of Bone Marrow-Derived Mesenchymal Stem Cells on Experimentally induced Schistosoma mansoni Related Liver Fibrosis.Int.J.Curr.Microbiol.App.Sci. 8(12): 1136-1151. doi: https://doi.org/10.20546/ijcmas.2019.812.143