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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(12): 1071-1081
DOI: https://doi.org/10.20546/ijcmas.2019.812.136


Phytochemical Screening and Antimicrobial Activity of Vitex negundo Leaf and Stem Extracts against Bacterial and Fungal Pathogens
Alka Bameta1*, Shruti Sanwal1 and Sonu Ambwani2
1Department of Biotechnology and Allied Science, M. B. Govt. P. G. College,
Haldwani- 263139, India
2Molecular Biology and Genetic Engineering College of Basic Sciences and Humanities, G.B. Pant University of Agriculture and Technology, Pantnagar- 263145, India
*Corresponding author
Abstract:

Vitex negundo (family: Lamiaceae) is a medicinal shrub which is known to exhibit curative activity against several ailments like relieve catarrh, headache, reduce inflammatory swellings of joints in rheumatic attacks etc. In the present study, the bioactive compounds present in leaf and stem of Vitex negundo were investigated for antimicrobial activity against some pathogenic bacteria (Bacillus subtilis, Staphylococcus aureus, Pseudomonas aeruginosa, and Salmonella typhi) and fungus (Asparagus niger, Cryptococcus neoformans, Saccharomyces cerevisiae, Candida albicans). Dried leaf and stem samples were used to obtain various extracts viz. methanol, ethanol, petroleum ether, hexane and chloroform. The phytochemical analyses showed the presence of phenol, steroids, saponins, coumarin and reducing sugar in the various extracts used in this study. In antibacterial study, leaf hexane extract showed the highest inhibition zone against Pseudomonas aeruginosa while stem ethanolic extract was found to be most effective against Salmonella typhi. The methanol and ethanol leaf extracts were best recorded for the antifungal activity against Cryptococcus neoformans. The stem chloroform extract has also showed maximum inhibition in case of Cryptococcus neoformans growth.


Keywords: Antibacterial, Antifungal, Extract, Phytochemicals, Vitex negundo
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How to cite this article:

Alka Bameta, Shruti Sanwal and Sonu Ambwani. 2019. Phytochemical Screening and Antimicrobial Activity of Vitex negundo Leaf and Stem Extracts against Bacterial and Fungal Pathogens.Int.J.Curr.Microbiol.App.Sci. 8(12): 1071-1081. doi: https://doi.org/10.20546/ijcmas.2019.812.136