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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(2): 1386-1398
DOI: http://dx.doi.org/10.20546/ijcmas.2017.602.157


Evaluation of Nutritional and Environmental Conditions for Phenol Degradation by a Lebanese strain of Candida tropicalis
Hiba Koubeissi*, Hoda Yusef and Hanafy Holail
Department of Biological and Environmental Sciences, Faculty of Science,Beirut Arab University, Debbieh, Lebanon
*Corresponding author
Abstract:

Phenol is a toxic hydrocarbon for nervous system, kidneys, liver, muscles and immune system. It is also mutagenic and possibly carcinogenic. Phenol containing- pollutants are dangerous and spread worldwide in the industrial effluents.The present study aimed to isolate a potent microorganism able to degrade phenol, as well as to evaluate the nutritional and environmental conditions that lead to its maximum degradation. A yeast isolated from petroleum-contaminated soil on MSM supplemented with phenol as sole carbon source, was identified by 28S rDNA sequencing. Residual phenol was estimated using 4-AAP method and the growth of yeast was measured spectrophotometrically at 600 nm. Candida tropicalis H was found to be highly effective for the removal of phenol. Phenol degradation was approximately 92.89 % of an initial concentration of 2000 mg phenol l-1 at 30°C in shaken culture (100 rpm) after 3 days incubation. After the application of Plackett-Burman design, 99.91 % degradation of phenol was achieved. The most effective factors influencing phenol degradation, among studied 11 variables, were K2HPO4 and culture volume. Candida tropicalis H could be a good candidate for the bioremediation of phenol contaminants in heavily polluted sites. A phytotoxicity study revealed that the biodegradation of phenol resulted in its detoxification, which indicated a possible use of phenol containing-effluents in the irrigation of plants after bioremediation process.


Keywords: Phenol biodegradation, Bioremediation, Candida tropicalis, Phenol measurement, Plackett-Burman
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How to cite this article:

Hiba Koubeissi, Hoda Yusef and Hanafy Holail. 2017. Evaluation of Nutritional and Environmental Conditions for Phenol Degradation by a Lebanese strain of Candida tropicalis.Int.J.Curr.Microbiol.App.Sci. 6(2): 1386-1398. doi: http://dx.doi.org/10.20546/ijcmas.2017.602.157