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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(12): 36-46
DOI: https://doi.org/10.20546/ijcmas.2019.812.006


Influence of Temperature, pH and Light on Growth and Sporulation of Alternaria solani (Ellis and Martin) Jones and Grout Causing Early Blight of Tomato (Solanum lycopersicum L.) under in vitro Condition
Rajendra Kumar Bais1, Ved Ratan1, Sumit Kumar2* and Somesh1
1Department of Plant Pathology, C.S.A. University of Agriculture and Technology, Kanpur,
Uttar Pradesh, India
2Department of Mycology and Plant Pathology, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh, India
*Corresponding author
Abstract:

Effect of different temperature, pH levels, and light intensity were tested against the growth of Alternaria solani under in vitro conditions. Mycelium growth and sporulation of Alternaria solani was significantly influenced with temperature. Maximum colony growth was recorded at 25oC followed by 30oC and minimum colony growth was recorded at 40oC. Excellent sporulation was noticed at 20oC and 25oC temperature. However, no sporulation was found at 40oC temperature. Effect of different pH level on colony diameter, sporulation and mycelium dry weight of Alternaria solani on PDA medium reveal that, the maximum colony diameter, dry weight were observed at 6.5 pH level followed by 6.0 pH level, while minimum colony diameter and dry weight were observed at 8.5 pH. Excellent sporulation was observed at pH level of 6.0 to 7.0, while poor sporulation was recorded at 4.0, 8.0 and 8.5 pH level. Light also influenced the colony growth and sporulation of Alternaria solani on PDA medium. Maximum colony growth and excellent sporulation was noticed at 12 hrs dark and 12 hrs light, minimum colony growth was observed at 8 hrs dark and 16 hrs light, good sporulation at 24 hrs light and 24 hrs dark and poor sporulation was observed at 8 hrs dark and16 hrs light and 8 hrs light and 16 hrs dark.


Keywords: Tomato, Mycelial growth, Fungal pathogen, Temperatures, pH levels, Light
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How to cite this article:

Rajendra Kumar Bais, Ved Ratan, Sumit Kumar and Somesh. 2019. Influence of Temperature, pH and Light on Growth and Sporulation of Alternaria solani (Ellis and Martin) Jones and Grout causing Early Blight of Tomato (Solanum lycopersicum L.) under In vitro condition.Int.J.Curr.Microbiol.App.Sci. 8(12): 36-46. doi: https://doi.org/10.20546/ijcmas.2019.812.006