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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(11): 2594-2601
DOI: https://doi.org/10.20546/ijcmas.2019.811.300


Molecular Detection of Peste des-petits Ruminant Virus (PPRV) by RT-PCR
Pragati Bhoyar, Prabhakar Tembhurne*, Ruchi Bhate, Prashant Tarale, Anuradha Nemade Anand Kadam, Jivan Kesharkar and V.C. Ingle
Department of Veterinary Microbiology and Animal Biotechnology, Nagpur Veterinary College, Nagpur, India
*Corresponding author
Abstract:

Peste des petits ruminants is one of the important transboundary, highly contagious and infectious viral disease of small ruminants especially sheep and goats caused by Small Ruminant Morbillivirus alias Peste- des- petits ruminants virus. In the current study, PPRV was detected in clinical samples of suspected goats as well as post vaccinated samples in nasal swabs and PBMC by conventional RT-PCR. Out of the 23 nasal swabs (12 infected and 11 vaccinated) 11 infected and one vaccinated samples was found positive. Out of 16 (5 infected and 11 vaccinated), all five infected and one vaccinated PBMC samples were found positive. Thus, the present study delineated the presence of PPRV in clinical samples as well as viaremic stages of PPRV. Further sequencing and characterization will warrant lineage of PPRV circulating in Nagpur district of Maharashtra.


Keywords: PPRV, RT-PCR, PBMC, Nasal Swab
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How to cite this article:

Pragati Bhoyar, Prabhakar Tembhurne, Ruchi Bhate, Prashant Tarale, Anuradha Nemade Anand Kadam, Jivan Kesharkar and Ingle, V.C. 2019. Molecular Detection of Peste des-petits ruminant virus (PPRV) by RT-PCR.Int.J.Curr.Microbiol.App.Sci. 8(11): 2594-2601. doi: https://doi.org/10.20546/ijcmas.2019.811.300