National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
SSR polymorphism in two rice varieties viz., CRMS31A (donor parent cytoplasmic male sterile line) and Aruna (high yielding, short duration variety with long, bold red grain. tolerant to BPH, medium resistant to stem borer and sheath root) was investigated to identify ready to use SSR markers which can be employed in selection of the marker assisted breeding programmes involving these varieties. A total of 115 simple sequence repeat (SSR) primers which were distributed on all the twelve chromosomes of rice were utilized. The results revealed distinct polymorphism among the cms and red rice parents for 20 RM primers. Based on this study, the large range of similarity and dissimilarity values for CRMS31A and Aruna provides greater confidence for the assessment of simple sequence repeat (SSR) polymorphism. Diversity analysis can be done by using these polymorphic SSR markers. In this study, PCR-based marker was used for distinguishing male sterile line from maintainer line. Results showed that cms marker had ability to differentiate male sterile line CRMS31A and Aruna (maintainer).This is helpful in promotion of hybrid seed production technology in Kerala state of India which is main consumer of bold-red rice.
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