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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(11): 2555-2563
DOI: https://doi.org/10.20546/ijcmas.2019.811.295


SSR Polymorphism Survey between CRMS31A and Aruna (MO8) for Marker Assisted Backcross Breeding in Rice
T.S. Lachyan1*, V.G. Jayalekshmy1 and Swapna Alex2
1 Department of Plant Breeding and Genetics,
2Department of Plant Biotechnology, College of Agriculture, Kerala Agricultural University, India
*Corresponding author
Abstract:

SSR polymorphism in two rice varieties viz., CRMS31A (donor parent cytoplasmic male sterile line) and Aruna (high yielding, short duration variety with long, bold red grain. tolerant to BPH, medium resistant to stem borer and sheath root) was investigated to identify ready to use SSR markers which can be employed in selection of the marker assisted breeding programmes involving these varieties. A total of 115 simple sequence repeat (SSR) primers which were distributed on all the twelve chromosomes of rice were utilized. The results revealed distinct polymorphism among the cms and red rice parents for 20 RM primers. Based on this study, the large range of similarity and dissimilarity values for CRMS31A and Aruna provides greater confidence for the assessment of simple sequence repeat (SSR) polymorphism. Diversity analysis can be done by using these polymorphic SSR markers. In this study, PCR-based marker was used for distinguishing male sterile line from maintainer line. Results showed that cms marker had ability to differentiate male sterile line CRMS31A and Aruna (maintainer).This is helpful in promotion of hybrid seed production technology in Kerala state of India which is main consumer of bold-red rice.


Keywords: Cytoplasmic male sterility, Polymorphic, Simple Sequence Repeats (SSR), Foreground selection, Background selection
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How to cite this article:

Lachyan, T.S., V.G. Jayalekshmy and Swapna Alex. 2019. SSR Polymorphism Survey between CRMS31A and Aruna (MO8) for Marker Assisted Backcross Breeding in Rice.Int.J.Curr.Microbiol.App.Sci. 8(11): 2555-2563. doi: https://doi.org/10.20546/ijcmas.2019.811.295