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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(2): 1287-1293
DOI: http://dx.doi.org/10.20546/ijcmas.2017.602.145


Comparison and Demonstration of Surface Keratin Staining Quality by Modified Papanicalou Stain, Modified Mallory’s Stain and Hematoxylin and Eosin Stain in Verrucous Carcinoma and Normal Keratinizing Epithelium
Santhosh Kumar Caliaperoumal1*, Sudhakar Srinivasan2 and Meer Ahmed Ibrahim Munshi3
1Department of Dentistry, Vinayaka Mission’s Medical College and Hospital,Karaikal, Puducherry, India
2Department of Dentistry, Karpagam Medical College and Hospital, Coimbatore, India
3Department of Oral surgery, Sri Ramakrishna Dental College and Hospital, Coimbatore, India
*Corresponding author
Abstract:

Observation of normal or abnormal keratin forms is the major criteria in histopathological diagnosis of Verrucous carcinoma. The aim of the study is to evaluate and compare the distinct staining and surface keratin in verrucous carcinoma and normal keratinizing epithelium by routine hematoxylin and eosin (H&E) stain, modified papanicolou stain and modified Mallory’s stain. The known cases of verrucous carcinoma and tissues from normal keratinizing epithelial regions with sufficient paraffin embedded tissue were selected. The three sections from each block were stained with H&E stain, modified Papanicolou stain and modified Mallory’s stain. The modified Mallory’s stain, modified Papanicolou stain distinctively and clearly stained surface keratin compared to H&E stain. The positive staining of surface keratin was significantly associated with all stains at the level of p=0.001 in verrucous carcinoma and normal epithelium respectively. Based on our observation we conclude that the positive staining of surface keratin was significantly associated at p=0.001 in verrucous carcinoma and normal epithelium with all stains however the positivity was more associated with modified Papanicolou stain followed by modified Mallory stain least by heamatoxylin and eosin Stain. So, these special stains can be used as an adjuvant stain along with H&E stain.


Keywords: Verrucous carcinoma (VC), Modified Papanicolou (MP) stain, Modified Mallory’s (MM) stain, Hematoxylin and Eosin (H&E) stain.
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How to cite this article:

Santhosh Kumar Caliaperoumal, Sudhakar Srinivasan and Meer Ahmed Ibrahim Munshi. 2017. Comparison and Demonstration of Surface Keratin Staining Quality by Modified Papanicalou Stain, Modified Mallory’s Stain and Hematoxylin and Eosin Stain in Verrucous Carcinoma and Normal Keratinizing Epithelium.Int.J.Curr.Microbiol.App.Sci. 6(2): 1287-1293. doi: http://dx.doi.org/10.20546/ijcmas.2017.602.145