Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2019) [Effective from January 1, 2019]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(11): 2187-2205
DOI: https://doi.org/10.20546/ijcmas.2019.811.254


Cow Side and Laboratory Tests for Diagnosis of Subclinical Mastitis in Cows
Aruna Maramulla1*, Ambica Gadige1, Lakshmi Kosapati2, Swathi Bommu3,Padmaja Katta4 and Ramesh Keshamoni1
1Department of Veterinary Medicine, 3Department of Veterinary Physiology and Biochemistry, College of Veterinary Science, Rajendranagar,Hyderabad – 500 030, Telangana, India
2Department of Veterinary medicine, College of Veterinary Science, Korutla – 505 326, Jagtial District, Telangana, India
4Department of Veterinary Medicine and Animal Husbandry Polytechnic, Mahaboobnagar - 509 001, Telangana, India
*Corresponding author
Abstract:

A total of 492 quarter milk samples from 123 animals were screened with CMT and among these, 366 quarter milk samples were negative for CMT reaction whereas, 40, 54 and 32 quarter milk samples showed +, ++ and +++ grades of CMT reactions with 74.39%, 8.13%, 10.98% and 6.50%, respectively. 492 milk samples were also analyzed for SCC and out of these 357, 33, 41, 18, 29 and 14 samples were showing SCC in the range of <1 lakh, 1-2 lakhs, 2-3 lakhs, 3-4 lakhs, 4-5 lakhs and 5-6 lakhs, respectively. Out of 492 quarter milk samples, 164 were found culturally positive and among these 87 (53.05%) positive for Staphylococcus spp., 22 (13.42%) for Streptococcus spp., 28 (17.07%) for Escherichia coli, 4 (2.44%) for Klebsiella spp., 5 (3.05%) for Staphylococcus spp. + Streptococcus spp., 5 (3.05%) for Escherichia coli + Streptococcus spp., and 13 (7.93%) for Escherichia coli + Staphylococcus spp. Comparative study of diagnostic tests was conducted between CMT, SCC and cultural examination and found animal wise incidence as 63.42, 66.67 and 73.98% respectively, whereas quarter wise incidence was 25.61, 27.44 and 33.33%, respectively. Among the CMT negative and 1+, 2+ and 3+ grades CMT positive samples, culturally positive samples were 54, 31, 48 and 31 with 14.75%, 77.50%, 88.89% and 96.86%, respectively. Whereas, culturally positive cases with regard to SCC of <1 lakh, 1-2 lakhs, 2-3 lakhs, 3-4 lakhs, 4-5 lakhs and 5-6 lakhs were 36, 28, 39, 18, 29 and 14 with 10.08, 84.85, 95.12, 100.00, 100.00 and 100.00 percent, respectively. The percent accuracy of CMT and SCC taking cultural examination as standard was 85.77 and 89.63 percent, whereas false positive are 12.70 and 11.11 percent and false negative results are 14.75 and 10.08 percent respectively. Sensitivity of CMT and SCC considering cultural examination as standard was reported as 67.07 and 76.92 percent, respectively and with specificity of 95.12 and 95.54 percent and predictive value of positive test as 87.30 and 88.89 percent, respectively.


Keywords: Sub clinical mastitis, Diagnosis, California mastitis test, Somatic cell count, Cultural examination
Download this article as Download

How to cite this article:

Aruna Maramulla, Ambica Gadige, Lakshmi Kosapati, Swathi Bommu, Padmaja Katta and Ramesh Keshamoni. 2019. Cow Side and Laboratory Tests for Diagnosis of Subclinical Mastitis in Cows.Int.J.Curr.Microbiol.App.Sci. 8(11): 2187-2205. doi: https://doi.org/10.20546/ijcmas.2019.811.254