National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
A total of 492 quarter milk samples from 123 animals were screened with CMT and among these, 366 quarter milk samples were negative for CMT reaction whereas, 40, 54 and 32 quarter milk samples showed +, ++ and +++ grades of CMT reactions with 74.39%, 8.13%, 10.98% and 6.50%, respectively. 492 milk samples were also analyzed for SCC and out of these 357, 33, 41, 18, 29 and 14 samples were showing SCC in the range of <1 lakh, 1-2 lakhs, 2-3 lakhs, 3-4 lakhs, 4-5 lakhs and 5-6 lakhs, respectively. Out of 492 quarter milk samples, 164 were found culturally positive and among these 87 (53.05%) positive for Staphylococcus spp., 22 (13.42%) for Streptococcus spp., 28 (17.07%) for Escherichia coli, 4 (2.44%) for Klebsiella spp., 5 (3.05%) for Staphylococcus spp. + Streptococcus spp., 5 (3.05%) for Escherichia coli + Streptococcus spp., and 13 (7.93%) for Escherichia coli + Staphylococcus spp. Comparative study of diagnostic tests was conducted between CMT, SCC and cultural examination and found animal wise incidence as 63.42, 66.67 and 73.98% respectively, whereas quarter wise incidence was 25.61, 27.44 and 33.33%, respectively. Among the CMT negative and 1+, 2+ and 3+ grades CMT positive samples, culturally positive samples were 54, 31, 48 and 31 with 14.75%, 77.50%, 88.89% and 96.86%, respectively. Whereas, culturally positive cases with regard to SCC of <1 lakh, 1-2 lakhs, 2-3 lakhs, 3-4 lakhs, 4-5 lakhs and 5-6 lakhs were 36, 28, 39, 18, 29 and 14 with 10.08, 84.85, 95.12, 100.00, 100.00 and 100.00 percent, respectively. The percent accuracy of CMT and SCC taking cultural examination as standard was 85.77 and 89.63 percent, whereas false positive are 12.70 and 11.11 percent and false negative results are 14.75 and 10.08 percent respectively. Sensitivity of CMT and SCC considering cultural examination as standard was reported as 67.07 and 76.92 percent, respectively and with specificity of 95.12 and 95.54 percent and predictive value of positive test as 87.30 and 88.89 percent, respectively.
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