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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Rotaviruses of the species A (RVA) are of particular epidemiological importance and recognized as being worldwide dispersed. RVA causes the majority of infections in humans, as well as are highly prevalent among domestic animals. Several studies have shown that zoonotic transmission may occur from animals to humans and this may account for the current broad antigenic and genetic rotavirus diversity, representing a potential important evolutionary mechanism. The present study aims to identify the evolutionary origins of circulating RVA strains in the Amazon region, using phylodynamics methods to assess and understand the relationship between hosts, inter-specific gene transfer and the chronology of viral infection. In total 83 RVA-positive faecal samples of human origin were analyzed, all of which obtained within the official Rotavirus Epidemiological Surveillance Network in Brazil. The genome of 17 RVA samples were selected and sequenced. Eight RVA strains of zoonotic origins were identified. Of the five G4P[6] genotype samples were shown to be of porcine origin, and two samples were generated from reassortment events involving genotypes of human and porcine strains. One sample of G3P[3] genotype strain, identify as lineage III, have evolved from canine, feline or simian origin, including a reassortment event with RVA strains from lineage II. In addition, G12P[9] strains had their origins in chiroptera, cattle or felines. The present study included phylodynamic analyses in order to elucidate those otherwise unknown evolutionary patterns, mainly in regards to G3P[3] and G12P[9] genotypes. There was a particular focus on the occurrence of reassortment events and the evolutionary mechanism which underlie the emergence of these strains. These evolutionary events may help in the monitoring of emergent strains of RVA with zoonotic potential and vaccine-escape possibility.