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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(11): 545-555
DOI: https://doi.org/10.20546/ijcmas.2019.811.067


Histological Study of the Possible Therapeutic Effect of Bone Marrow Derived Mesenchymal Stem Cells on the Ultrastructure of the Corneal Keratocytes in Alkali Burn Model in Adult Male Albino Rats
Suzan E. Abo Elnasr*, Shereen Sh. El Abd, Sadika M. El-Ebs, Gehan M. Soliman and Naglaa I. Sarhan
Department of Histology, Faculty of Medicine, Tanta University, Egypt
*Corresponding author
Abstract:

Chemical burnsare serious ocular emergencies that require immediate interventionto minimize severe complications and visual loss. Keratocytes (corneal corpuscles) are the main cells within the corneal stroma that can be activated after injury generating myofibroblast precursors. Previous studies showed that bone marrow mesenchymal stem cells (BM-MSCs) have potential therapeutic value in corneal reconstruction. This work was performed to evaluate the ultrastructural changes of the corneal keratocytes due to alkali burn and the possible therapeutic effect of bone marrow derived mesenchymal stem cells (BM-MSCs).Thirty adult male albino rats were used and divided equally into three main groups:Group I: was the control group. Group II: alkali burn was induced and left without any treatment. Group III: alkali burn was induced and rats were treated with single subconjunctival injection of 2.4×106BM-MSCs.In each group, half of the animals was sacrificed after one week while the other half was sacrificed after three weeks. Electron microscopic examination of corneal keratocytes of group II revealed marked alteration in the form of multiple cytoplasmic vacuoles and increase in their size in addition to nuclear indentation, condensation, bizzare shape and eccentric position. On the other hand, the stem cell treated group showed disappearance of most of these histological alternations. From the present study it could be concluded that corneal Alkali burn stimulated keratocytesto undergo cell death and to lose their quiescence state and BM-MSCs could effectively promote corneal alkali burn healing through improvement of corneal keratocytes.


Keywords: bone marrow derived mesenchymal stem cells, Keratocytes, Chemical injuries
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How to cite this article:

Suzan E. Abo Elnasr, Shereen Sh. El Abd, Sadika M. El-Ebs, Gehan M. Soliman and Naglaa I. Sarhan 2019. Histological Study of the Possible Therapeutic Effect of Bone Marrow Derived Mesenchymal Stem Cells on the Ultrastructure of the Corneal Keratocytes in Alkali Burn Model in Adult Male Albino Rats.Int.J.Curr.Microbiol.App.Sci. 8(11): 545-555. doi: https://doi.org/10.20546/ijcmas.2019.811.067