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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(2): 1214-1224
DOI: http://dx.doi.org/10.20546/ijcmas.2017.602.138


In silico Identification and Characterization of Conserved miRNAs and their Targets in Pigeon pea (Cajanus cajan L.) Expressed Sequence Tags
Akula Dinesh1* and More Yogeshwar2
1Department of Genetics and Plant Breeding, UAS, Raichur, 584102, India
2Department of Plant Pathology; PDKV, Akola, Maharastra, India
*Corresponding author
Abstract:

MicroRNAs (miRNAs) are highly conserved class of short endogenous non-coding small RNA molecules of about 18–22 nucleotide in length. MicroRNAs negatively regulate the gene expression by degrading target mRNA. In the present study, comparative genomic based approach was used to identify and characterize new conserved microRNAs in “orphan legume crop” pigeon pea (Cajanus cajan L.) using expressed sequence tags (ESTs) by in silico method. A total of 4,621 unique previously reported microRNAs were used for homology search in 25,576 ESTs of pigeon pea for identifying conserved miRNAs. The results upon stringent selection found five conserved miRNAs namely cca-miR6483, cca-miR5219, cca-miR393a, cca-miR395a, cca-miR169b belonging to five different families. The target analysis through psRNATarget server found 27 mRNA targets which code for eukaryotic translation initiation factor 6 (EIF-6)-like protein, apyrase-like protein, ferric reductase, ATP sulfurylase, CCAAT-box transcription factor complex WHAP12, Lipoxygenase-9, Transport inhibitor response 1, and MYB transcription factor MYB102 which play an important role in response to both biotic and abiotic stresses. Our results have laid the foundation for further research on miRNAs, which will lead to understand the gene silencing mechanisms at post transcriptional level for various stresses.


Keywords: MicroRNA,Pigeon pea,EST, Gene expression,In silico.
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How to cite this article:

Akula Dinesh and More Yogeshwar. 2017. In silico Identification and Characterization of Conserved miRNAs and their Targets in Pigeon pea (Cajanus cajan L.) Expressed Sequence TagsInt.J.Curr.Microbiol.App.Sci. 6(2): 1214-1224. doi: http://dx.doi.org/10.20546/ijcmas.2017.602.138