Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2019) [Effective from January 1, 2019]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(11): 2000-2025
DOI: https://doi.org/10.20546/ijcmas.2019.811.234


Evaluation of Antimicrobial Activity of ZnO Nanoparticles against Foodborne Pathogens
Anjie Jamal1*, Ramadan Awad1 and Hoda Yusef2
1Department of Biological Sciences, Faculty of Science, Beirut Arab University, Lebanon
2Department of Botany and Microbiology, Faculty of Science, Alexandria University, Egypt
*Corresponding author
Abstract:

Nanostructures have a great potential in the area of food packaging. This study aims to determine the antimicrobial efficacy for zinc oxide (ZnO) nanoparticles (Nps), compared to bulk ZnO powder. ZnO Nps were synthesized using; co-precipitation and high-speed ball milling method. The synthesized powders were characterized by X-ray diffraction (XRD), Transmission Electron Microscope (TEM), Fourier Transform Infrared Spectroscopy (FTIR) and Ultraviolet-Visible Absorption Spectroscopy (UV). All data revealed the formation of pure nano-sized ZnO. ZnO Nps showed notable size and concentration-dependent antimicrobial effects. In comparison to nanosuspension, the antimicrobial activity of bulk ZnO was almost negligible, a strong significant difference was noticed between the antimicrobial activity of the bulk and that of prepared Nps (p<0.05). ZnO Nps exhibited a privileged ability to suppress the growth of foodborne pathogens in culture media and milk samples. Among the bacterial strains tested, Gram-positive bacteria were more sensitive to ZnO nano-treatments. The examination of DNA of Bacillus subtilis treated with ZnO Nps using gel electrophoresis, displayed a reduction in band size but the absence of DNA fragmentation. Transmission Electron Microscope illustrated remarkable damages in the cell wall and cytoplasmic membrane of Bacillus subtilis cell structure upon exposure to ZnO Nps.


Keywords: ZnO Nanoparticles, Antimicrobial activity, Foodborne pathogens
Download this article as Download

How to cite this article:

Anjie Jamal, Ramadan Awad and Hoda Yusef. 2019. Evaluation of Antimicrobial Activity of ZnO Nanoparticles against Foodborne Pathogens.Int.J.Curr.Microbiol.App.Sci. 8(11): 2000-2025. doi: https://doi.org/10.20546/ijcmas.2019.811.234