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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(11): 1839-1848
DOI: https://doi.org/10.20546/ijcmas.2019.811.216


Frequency and Distribution of rph2 Gene in Tribolium castaneum Collected from Grain Supply Chain of Coimbatore, Kangeyam and Theni
C. Taraka and S. Mohankumar*
Department of Plant Biotechnology, Tamil Nadu Agricultural University, Coimbatore-641003, India
*Corresponding author
Abstract:

Resistance to insecticides is a worldwide issue for the major stored insect pests. Among several insecticides, phosphine (PH3) gas is the most commonly used fumigant to protect the stored product from pests. Conventionally, a discriminating-dose bioassay is conducted to determine the phosphine resistance in stored insect pests. In the present study, the frequency of resistance is estimated in Tribolium castaneum (Herbst) populations collected from grain supply chain viz., (Grain processing unit, bulk grain storage godowns, ration shop, wholesale shop, retail shop and household) from three locations of Tamil Nadu, India. Based on a cleaved amplified polymorphic sequence (CAPS) method, each individual insect is analysed using restriction enzyme to detect strong resistance allele. Among the populations collected from grain supply chain of Theni, Coimbatore and Kangeyam, the samples (T. castaneum) from bulk grain storage showed the highest frequency of phosphine resistant allele of viz., 85.71, 78.33, 89.13%, respectively while as the lowest frequency of phosphine resistant allele was observed in household samples of Theni, Coimbatore and Kangeyam viz.,18.75, 34.48, 40.74%, respectively. Thus, strong phosphine resistance detected in beetle with the help of CAPS marker could be used for the improvement of management strategies for the given pest populations.


Keywords: Tribolium castaneum, Phosphine resistance, rph2 gene, CAPS marker
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How to cite this article:

Taraka, C. and Mohankumar, S. 2019. Frequency and Distribution of rph2 Gene in Tribolium castaneum Collected from Grain Supply Chain of Coimbatore, Kangeyam and Theni.Int.J.Curr.Microbiol.App.Sci. 8(11): 1839-1848. doi: https://doi.org/10.20546/ijcmas.2019.811.216