National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Resistance to insecticides is a worldwide issue for the major stored insect pests. Among several insecticides, phosphine (PH3) gas is the most commonly used fumigant to protect the stored product from pests. Conventionally, a discriminating-dose bioassay is conducted to determine the phosphine resistance in stored insect pests. In the present study, the frequency of resistance is estimated in Tribolium castaneum (Herbst) populations collected from grain supply chain viz., (Grain processing unit, bulk grain storage godowns, ration shop, wholesale shop, retail shop and household) from three locations of Tamil Nadu, India. Based on a cleaved amplified polymorphic sequence (CAPS) method, each individual insect is analysed using restriction enzyme to detect strong resistance allele. Among the populations collected from grain supply chain of Theni, Coimbatore and Kangeyam, the samples (T. castaneum) from bulk grain storage showed the highest frequency of phosphine resistant allele of viz., 85.71, 78.33, 89.13%, respectively while as the lowest frequency of phosphine resistant allele was observed in household samples of Theni, Coimbatore and Kangeyam viz.,18.75, 34.48, 40.74%, respectively. Thus, strong phosphine resistance detected in beetle with the help of CAPS marker could be used for the improvement of management strategies for the given pest populations.
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