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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(11): 1802-1811
DOI: https://doi.org/10.20546/ijcmas.2019.811.211


Antibiogram and Genotyping of Extended-Spectrum β-lactamase (ESBL) Producing Escherichia coli from Poultry
S.N. Ghodasara1*, A.R. Baria2, A.S. Patel2 and B.B. Javia1
1Department of Veterinary Microbiology, College of Veterinary Science and A. H., Junagadh Agricultural University, Junagadh, Gujarat, India
2Department of Livestock Products Technology, College of Veterinary Science and A. H., Junagadh Agricultural University, Junagadh, Gujarat, India
*Corresponding author
Abstract:

In the present study, total 100 cloacal/intestinal swabs from poultry were collected from the retail chicken meat shop from the city of Junagadh, Gujarat. Of this, total 80 (80%) isolates were identified morphologically and biochemically as E. coli. The antibiotic resistance patterns of all these isolates were studied using disc diffusion method and showed 97.5% isolates were resistant to ceftriaxone/ salbactum followed by cefotaxime/clavulanate (96.25%), cefoxitin/cloxacillin (92.5%), cefixime (92.5%) and ceftizoxime (92.5%).Total 13 (16.25%) isolates were found phenotypically ESBL producing E. coli. Of these, 4 isolates shown difference of ≥5 mm zone of inhibition between cefotaxime and cefotaxime + clavulanate discs, 10 isolates shown difference of ≥5 mm zone of inhibition between ceftriaxone and ceftriaxone + salbactum discs and 3 isolates shown difference of ≥5 mm zone of inhibition between ceftazidime and ceftazidime + clavulanate discs and 28 isolates were found positive for the presence of blaTEM gene (445 bp) using molecular based detection, whereas none of the isolates shown desired amplification to blaSHV and blaCTX-M genes, indicating blaTEM gene is the most abundant ESBLs type prevails in this region.


Keywords: Phenotypically, Chicken meat shop, Diffusion method, Antibiotic resistance, ESBLs
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How to cite this article:

Ghodasara, S.N., A.R. Baria, A.S. Patel and Javia, B.B. 2019. Antibiogram and Genotyping of Extended-Spectrum β-lactamase (ESBL) Producing Escherichia coli from Poultry.Int.J.Curr.Microbiol.App.Sci. 8(11): 1802-1811. doi: https://doi.org/10.20546/ijcmas.2019.811.211