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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(11): 1404-1409
DOI: https://doi.org/10.20546/ijcmas.2019.811.164


Comparative Efficiency of Whole blood and FTA Card for Diagnosis of Theileriosis in Buffaloes of Nagpur Region, India
Sabhyata Sharma*, S.W. Kolte, N.V. Kurkure, S.G. Jadhao and Swapnil Magar
Department of Veterinary Parasitology, Nagpur Veterinary College, MAFSU, Nagpur, India
*Corresponding author
Abstract:

Theileriosis is a tick borne haemoprotozoan disease caused by Theileria annulata and Theileria orientalis, mainly. The disease is known to be highly fatal and is also responsible for heavy economical loses in dairy industry. In the present study, blood samples were collected from the buffaloes suspected for theileriosis. A total of 50 blood samples from jugular vein of buffaloes were collected in EDTA tube and a drop of fresh blood was also taken on FTA card for DNA isolation. For Polymerase chain reaction, the primer sequences were used as per Kakati et al., and Kolte et al., The PCR assay employs primers specific for the gene encoding 30kDa major merozoite surface antigen of Theileria annulata and major piroplasm surface protein gene of Theileria orientalis. Out of total 50 blood samples, 13 were positive for Theileria annulata by both whole blood PCR and FTA card PCR whereas for Theileria orientalis, 23 were positive by whole blood PCR and 27 were found positive by FTA card PCR. It was found that diagnosis of Theileriosis by using FTA card is highly recommended as it is sensitive, less laborious, less chances of DNA degradation, easy and quickest method of diagnosis as compared to Whole blood.


Keywords: Haemaphysalis, Ambylommaand Rhipicephalus, Theileria orientalis, Haemoprotozoan
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How to cite this article:

Sabhyata Sharma, S.W. Kolte, N.V. Kurkure, S.G. Jadhao and Swapnil Magar. 2019. Comparative Efficiency of Whole blood and FTA Card for Diagnosis of Theileriosis in Buffaloes of Nagpur Region, India.Int.J.Curr.Microbiol.App.Sci. 8(11): 1404-1409. doi: https://doi.org/10.20546/ijcmas.2019.811.164