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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(11): 1279-1286
DOI: https://doi.org/10.20546/ijcmas.2019.811.151


In Vitro Culture of Elite Sugarcane Cultivar (Saccharum spp) by Shoot Apical Meristem and Effect of Plant Hormones on Shoot Regeneration
R. Anitha1*, M. Shanmuganathan2 and M. Jayachandran3
1Department of Crop Physiology
2Department of Plant Breeding and Genetics
3Department of Agronomy, Sugarcane Research Station, Cuddalore
Tamil Nadu Agricultural University, Coimbatore, India
*Corresponding author
Abstract:

The present investigation was carried out at Tissue Culture Laboratory located in Sugarcane Research Station, Cuddalore on in vitro shoot regeneration of sugarcane apical meristem during the period of 2018-2019. The regeneration of sugarcane variety CoC25 with supplementation of cytokinin 0.1mg/l BAP, 0.015mg/lkin, 0.5mg/lGA3 was used in sugarcane on defined MS media for shoot induction. The concentration of 0.25mg/lBAP, 0.5mg/lkin,1.0mg/lGA3, 0.5mg/l NAA involved in shoot regeneration in variety CoC25 showed the highest shoot length, number of tillers and chlorophyll content. The concentration of ½ MS, 4mg/l NAA and 40g/l sucrose as only auxin involved in root initiation showed the highest number of roots and root length. The sugarcane plantlets were acclimatized in greenhouse before planting in the field.


Keywords: Auxin, Cytokinin, in vitro, Apical meristem, Shoot regeneration
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How to cite this article:

Anitha, R., M. Shanmuganathan and Jayachandran, M. 2019. In Vitro Culture of Elite Sugarcane Cultivar (Saccharum spp) by Shoot Apical Meristem and Effect of Plant Hormones on Shoot Regeneration.Int.J.Curr.Microbiol.App.Sci. 8(11): 1279-1286. doi: https://doi.org/10.20546/ijcmas.2019.811.151