National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Pharmacological control of the corpus luteum is significant in the control of the estrous cycle in female buffaloes. The corpus luteum experiences broad cell expansion and separation during luteinization and involutes quickly at luteolysis. Vascular endothelial growth factor (VEGF) and Fibroblast Growth factor (FGF 2) is essential for the angiogenesis required for the formation of the corpus luteum. The aim of this study was to determine the role of VEGF and FGF 2 on cell viability and apoptosis in corpus luteum buffalo. For this purpose, mid stage luteal cell were cultured. The cultured luteal cells were treated with VEGF A (100ng/ml) and FGF 2 (50ng/ml) for 72 hrs. At the end of the respective incubation periods, viability of cells was done by MTT assay, whereas the cultured cells were used for Caspase 3 mRNA expression by quantitative real-time polymerase chain reaction. The results indicated a significant increase in cell viability in the treatment groups as compared to control. The mRNA expression of Caspase 3 was significantly down regulated in the treatment group in comparison to control groups. Thus, the present finding shows a cell proliferative response of VEGF A and FGF 2 which indicates that these proteins have important role in CL development and function in buffaloes.
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