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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(11): 1238-1244
DOI: https://doi.org/10.20546/ijcmas.2019.811.145


Role of VEGF A and FGF 2 in Cell Viability and Apoptosis in Cultured Bubaline Luteal Cells
Meeti Punetha1, Avishek Paul1, Sai Kumar1, Bosco Jose1, Jaya Bharatiand Mihir Sarkar1*
1ICAR-Indian Veterinary Research Institute, Physiology & Climatology Division, Izatnagar, Bareilly, Uttar Pradesh-243122, India
2ICAR-National Research Center on Pig, Rani, Guwahati, Assam 781131, India
*Corresponding author
Abstract:

Pharmacological control of the corpus luteum is significant in the control of the estrous cycle in female buffaloes. The corpus luteum experiences broad cell expansion and separation during luteinization and involutes quickly at luteolysis. Vascular endothelial growth factor (VEGF) and Fibroblast Growth factor (FGF 2) is essential for the angiogenesis required for the formation of the corpus luteum. The aim of this study was to determine the role of VEGF and FGF 2 on cell viability and apoptosis in corpus luteum buffalo. For this purpose, mid stage luteal cell were cultured. The cultured luteal cells were treated with VEGF A (100ng/ml) and FGF 2 (50ng/ml) for 72 hrs. At the end of the respective incubation periods, viability of cells was done by MTT assay, whereas the cultured cells were used for Caspase 3 mRNA expression by quantitative real-time polymerase chain reaction. The results indicated a significant increase in cell viability in the treatment groups as compared to control. The mRNA expression of Caspase 3 was significantly down regulated in the treatment group in comparison to control groups. Thus, the present finding shows a cell proliferative response of VEGF A and FGF 2 which indicates that these proteins have important role in CL development and function in buffaloes.


Keywords: Buffalo, Corpus luteum, VEGF A, FGF 2
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How to cite this article:

Meeti Punetha, Avishek Paul, Sai Kumar, Bosco Jose, Jaya Bharati and Mihir Sarkar. 2019. Role of VEGF A and FGF 2 in Cell Viability and Apoptosis in Cultured Bubaline Luteal Cells.Int.J.Curr.Microbiol.App.Sci. 8(11): 1238-1244. doi: https://doi.org/10.20546/ijcmas.2019.811.145