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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(2): 1123-1128
DOI: http://dx.doi.org/10.20546/ijcmas.2017.602.126


AmpC Beta Lactamase Detection in Escherichia coli and Comparison of Two Phenotypic Methods
R. Manasi1* and K. B. Anitha2
1Department of Microbiology, Pariyaram Medical College, Pariyaram, India
2Department of Microbiology, A.J. Institute of Medical Sciences, Mangalore, India
*Corresponding author
Abstract:

Increasing  drug resistance due to the production of AmpC β-lactamases and Extended spectrum β-lactamases (ESBLs)  is  an  increasing  cause  of  concern  as  it  has  left  the  clinicians  with  limited  therapeutic  options. Aims were detection  of  AmpC β  lactamase  in  E. coli an comparison of the two phenotypic tests- AmpC   disk  test  and  Inhibitor  based   test  using  phenyl  boronic  acid. A total of 156 E. coli isolates from various clinical samples were collected for the study. These isolates were further subjected to ESBL detection by disc diffusion test, screening for AmpC β-lactamases by cefoxitin disc and confirmation of AmpC β-lactamases by AmpC disc test and Inhibitor based test using phenyl boronic acid. Statustical analysis done by Chi-Square test and Fisher’s Exact test. AmpC screening detected 68 isolates as presumptive AmpC producers, of which 82.4% were confirmed to be true AmpC producers by confirmatory tests. Phenotypic confirmation by AmpC disc test and Inhibitor based test detected 20.5% and 35.9% isolates respectively as AmpC producers. Coexistence of AmpC with ESBL was seen among 32(20.5%) isolates. Pure AmpC producers were 15.4%. Pure AmpC producers as well as co-producers of ESBL and AmpC showed multidrug resistance. Pure AmpC producers showed 100% sensitivity to cefepime and imipenem while co-producers showed only 25% sensitivity to cefepime and 87.5% sensitivity to imipenem. The prevalence of AmpC producers among E. coli in our study was 35.9%. Pure AmpC producers are 100% susceptible to cefepime and imipenem. The Inhibitor based test is a simple, efficient and better test for the detection of AmpC production.


Keywords: AmpC β lactamase, Inhibitor based test, AmpC disc test.
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How to cite this article:

Manasi, R., and Anitha, K.B. 2017. AmpC Beta Lactamase Detection in Escherichia coli and Comparison of Two Phenotypic Methods.Int.J.Curr.Microbiol.App.Sci. 6(2): 1123-1128. doi: http://dx.doi.org/10.20546/ijcmas.2017.602.126