National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Cases of onycomycosis have increased over the past few decades. In the last few years, a number of newer less toxic antifungal drugs have become available for clinical use. The increased use of antifungal, often for prolonged periods, has led to the recognition of the phenomenon of acquired antifungal resistance among previously susceptible strains or species and to the increased incidence of infections with less common species. So, there is an increasing need for rapid and accurate method for antifungal susceptibility testing. In the present study antifungal susceptibility testing was done by in-vitro micro broth dilution method according to CLSI guideline (M38-A). 25 confirmed Trichophyton rubrum of Nails sample collected from onycomycosis patients of suspected dermatophytosis from Delhi (India). To check Minimal inhibitory concentration (MIC) was performed in micro-titer plates with U-bottom and incubated at 35° C. Reading were taken after 96 hrs of incubation for Trichophyton rubrum, against 6 antifungal drugs namely fluconazole, itraconazole (triazoles), terbinafine, griseofulvin, luliconazole and voriconazole. Most of the dermatophytes had uniform patterns of susceptibility to the antifungal agents tested. Low MIC values as 64µg/ml and 0.03μg/mL were found for 52%, 64%, 72%, 88%, 100% and 100% of isolates for fluconazole, itraconazole, terbinafine, griseofulvin, luliconazole and voriconazole respectively.
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