Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2019) [Effective from January 1, 2019]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(11): 958-966
DOI: https://doi.org/10.20546/ijcmas.2019.811.112


Study of Clinico-Mycological and Molecular Profile and Antifungal Susceptibility Pattern of Dermatophytic Onycomycosis Infection in East Delhi, India
Shyama Datt*, Shukla Das and Thakur Datt
Department of Microbiology, UCMS and GTB Hospital, Dilshad Garden, Delhi-95, India
*Corresponding author
Abstract:

Cases of onycomycosis have increased over the past few decades. In the last few years, a number of newer less toxic antifungal drugs have become available for clinical use. The increased use of antifungal, often for prolonged periods, has led to the recognition of the phenomenon of acquired antifungal resistance among previously susceptible strains or species and to the increased incidence of infections with less common species. So, there is an increasing need for rapid and accurate method for antifungal susceptibility testing. In the present study antifungal susceptibility testing was done by in-vitro micro broth dilution method according to CLSI guideline (M38-A). 25 confirmed Trichophyton rubrum of Nails sample collected from onycomycosis patients of suspected dermatophytosis from Delhi (India). To check Minimal inhibitory concentration (MIC) was performed in micro-titer plates with U-bottom and incubated at 35° C. Reading were taken after 96 hrs of incubation for Trichophyton rubrum, against 6 antifungal drugs namely fluconazole, itraconazole (triazoles), terbinafine, griseofulvin, luliconazole and voriconazole. Most of the dermatophytes had uniform patterns of susceptibility to the antifungal agents tested. Low MIC values as 64µg/ml and 0.03μg/mL were found for 52%, 64%, 72%, 88%, 100% and 100% of isolates for fluconazole, itraconazole, terbinafine, griseofulvin, luliconazole and voriconazole respectively.


Keywords: Dermatophytes; Broth micro-dilution method; Minimal Inhibitory Concentration (MIC)
Download this article as Download

How to cite this article:

Shyama Datt, Shukla Das and Thakur Datt. 2019. Study of Clinico-Mycological and Molecular Profile and Antifungal Susceptibility Pattern of Dermatophytic Onycomycosis Infection in East Delhi, India.Int.J.Curr.Microbiol.App.Sci. 8(11): 958-966. doi: https://doi.org/10.20546/ijcmas.2019.811.112