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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
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Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(1): 174-189
DOI: http://dx.doi.org/10.20546/ijcmas.2016.501.015


Study the Genetic Variations Related to the Resistance of Heavy Metals Toxicity in Some Rice Genotypes Using RAPD Markers
S. A. A. Heiba, A.A.El-Mouhamady, Sara E. I. Eldessouky, Hoda B. M. Ali and T.A. Elewa
Department of Genetics and Cytology, Division of Genetic Engineering and Biotechnology, National Research Centre, Dokki, Giza, Egypt
Field crops Research Dept., Agriculture and Biological Division, National Research Center, Dokki, Giza, Egypt
*Corresponding author
Abstract:

Six genotypes of rice highly tolerance for salinity and drought, while different in terms of response for resistance of heavy metals namely,(P1= Sakha105),(P2= Sakha 106),(P3= Giza 179), (P4= GZ842312-9-4-5-1),(P5= IR 75) and (P6= IR 60), were used in this study through hybridization among them using half diallel analysis to check their ability to reduce the accumulation of heavy metals in leaves. Some characters were studied in this experiment, e.g. heading date, Plant height, Number of filled grains/panicle, 1000-grain weight, grain yield per plant, Cadmium , Copper , Cobalt , and Zinc Contents, in addition to genetic parameters, such as combining ability effects and heterosis over better –parent which were the measurements of genetic calculated through genetically and statistical analysis. RAPD-PCR using seven primers has been performed to investigate the phylogenetic relationships among the six parents and their four hybrids (The highest and lowest two values for the performance of characters) namely, H1 (Sakha 105 X Sakha 106), H2 (Sakha 106 X IR75), H3 (Giza 179 X IR60) and H4 (GZ842312-9-4-5-1X IR 75) respectively. It could be concluded that H4 was the high hybrid and the four parents, P1, P2, P3 and P5 seems to be the most suitable for it. Through the final review of the results for the averages observed that the following genotypes,(Sakha 106), (GZ842312-9-4-5-1),(IR 75),( (Giza 179 X IR 60) and (GZ842312-9-4-5-1X IR 75) were more resistant to the accumulation of such heavy metals and gave carries a high tolerance of the plants of rice that would increasing the resistance of the risks of the environmental stresses as well as reducing the toxicity.


Keywords: Rice, Half Diallel, GCA, SCA, Heterosis, Heavy metals and RAPD markers
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How to cite this article:

Int.J.Curr.Microbiol.App.Sci. 5(1): 174-189. doi: http://dx.doi.org/10.20546/ijcmas.2016.501.015