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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(11): 375-381
DOI: https://doi.org/10.20546/ijcmas.2019.811.047


Inter Simple Sequence Repeats (ISSR) Marker based Genetic Diversity Analysis in White Yam (Dioscorea rotundata Poir.)
Parvathy Harikumar* and M.N. Sheela
Department of Crop Improvement, ICAR-Central Tuber Crops Research Institute, Thiruvananthapuram, Kerala, India
*Corresponding author
Abstract:

Diversity in crops provides opportunity for plant breeders to develop new and improved cultivars with desirable characteristics, genetic improvement for existing varieties thus meet subsistence food requirement. DNA marker is reliable in genetic diversity study due to variations at sequence level, not influenced by environment and its expression in all tissues. Marker used in our study is Inter Simple Sequence Repeats (ISSR) for 30 different accessions of white yam including dwarf, semi dwarf and tall. Cluster analysis was done based on Euclidean distance of 30 accessions for ISSR, Dendrogram showed the partition of most of the dwarf and tall genotypes in to two different clusters and semi dwarf comes under the dwarf cluster. The ISSR primers produced an average of six polymorphic alleles with a mean Hobs and PIC values of 0.738 and 0.707 respectively. The higher PIC and Hobs values obtained in the present study for ISSR markers indicate high variability of population studied. Hence, ISSR markers can be used as a strong tool in elucidating genetic diversity in yams. Present study also identified a high yielding (8 kg/plant), highly divergent white yam genotype named DR17 that could be used for genetic improvement of white yam for future population.


Keywords: White yam, ISSR Markers, Genetic diversity, Euclidean distance, Hobs value and PIC value
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How to cite this article:

Parvathy Harikumar and Sheela, M.N. 2019. Inter Simple Sequence Repeats (ISSR) Marker based Genetic Diversity Analysis in White Yam (Dioscorea rotundata Poir.).Int.J.Curr.Microbiol.App.Sci. 8(11): 375-381. doi: https://doi.org/10.20546/ijcmas.2019.811.047