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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(11): 368-374
DOI: https://doi.org/10.20546/ijcmas.2019.811.046


Biophysical Characterization of Chemically Synthesized Antimicrobial Peptide ‘Mastoparan’ and Evaluation of its Activity on Staphylococcus aureus Isolated from Mastitic Milk
Rajkumar James Singh*, Sameer Shrivastava, Sonal, Pallavi Shah, Purnima Gogoi, Saumya Srivastava, Shahid Hussain, Deepanker Bisht, Shantanu Kodape, Rajeshwar Khandare and Raj Kumar Singh
Department of Veterinary Biotechnology, ICAR- Indian Veterinary Research Institute, Izatnagar, Bareilly-243122- India
*Corresponding author
Abstract:

A well-documented antimicrobial peptide, Mastoparan, initially isolated from the venom of wasp was chemically synthesized using solid phase peptide synthesis methodology. A total of 20 S. aureus isolates were isolated from milk samples. Antimicrobial sensitivity of these isolates against 9 (nine) conventional antibiotics, as well as, Mastoparan was studied. Majority of these isolates were resistant to many antibiotics. Mastoparan was observed to exhibit potent antimicrobial activity, even against some antibiotic-resistant isolates. Chloramphenicol was found to be most effective, whereas Ampicillin was least effective against S. aureus of milk origin.


Keywords: Antimicrobial peptide, Mastoparan, S. aureus, Antibiogram
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How to cite this article:

Rajkumar James Singh, Sameer Shrivastava, Sonal, Pallavi Shah, Purnima Gogoi, Saumya Srivastava, Shahid Hussain, Deepanker Bisht, Shantanu Kodape, Rajeshwar Khandare and Raj Kumar Singh. 2019. Biophysical Characterization of Chemically Synthesized Antimicrobial Peptide ‘Mastoparan’ and Evaluation of its Activity on Staphylococcus aureus Isolated from Mastitic Milk.Int.J.Curr.Microbiol.App.Sci. 8(11): 368-374. doi: https://doi.org/10.20546/ijcmas.2019.811.046