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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(10): 2703-2712
DOI: https://doi.org/10.20546/ijcmas.2019.810.311


Comparison of Polymerase Chain Reaction- Restriction Fragment Length Polymorphism (PCR-RFLP) and Repetitive Sequence-PCR (REP-PCR) for Differentiation of Riemerella anatipestifer Isolates
C. Devigasri1*, P. M. Priya1*, M. Mini1, Surya Sankar1 and T. V. Aravindhakshan2
1Department of Veterinary Microbiology, College of Veterinary and Animal Sciences, Mannuthy, Thrissur, Kerala, India 2Department of Animal Breeding Genetics and Biostatistics, College of Veterinary and Animal Sciences, Mannuthy, Kerala, India
*Corresponding author
Abstract:

Riemerella anatipestifer, a pathogen causing infectious serositis and septicaemia in ducks, turkey, geese, pheasants, swans, poultry and also reported in pigs, which belongs to family Flavobacteriaceae. Twenty one serotypes have been reported so far by serum agglutination test which is found to be labour intensive and time consuming. In order to find out the better method to differentiate the isolates, PCR-RFLP and REP-PCR has been compared. The 20 isolates of R. anatipestifer were subjected to 16S rRNA PCR and subjected to restriction digestion with Hae III and the banding pattern was observed for differentiation. On amplifying the repetitive extragenic palindromic sequences of R. anatipestifer, the electrophoretic patterns of PCR products obtained were considered for the classification. In both methods, the differences in banding pattern were analysed using PyElph 4.1 software for dendrogram construction. Both methods differentiated the isolates into three groups whereas REP-PCR was found to be the most efficient method owing to its easiness and repeatability. Hence, REP-PCR could be used for epidemiological investigation and characterisation of R.anatipestifer isolates.


Keywords: Riemerella anatipestifer, PCR-RFLP, REP-PCR, Electrophoretic patterns
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How to cite this article:

Devigasri C., P. M. Priya, M. Mini, Surya Sankar and Aravindhakshan T. V. 2019. Comparison of Polymerase Chain Reaction- Restriction Fragment Length Polymorphism (PCR-RFLP) and Repetitive Sequence-PCR (REP-PCR) for Differentiation of Riemerella anatipestifer Isolates.Int.J.Curr.Microbiol.App.Sci. 8(10): 2703-2712. doi: https://doi.org/10.20546/ijcmas.2019.810.311