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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(2): 997-1003
DOI: http://dx.doi.org/10.20546/ijcmas.2017.602.112


Optimization for Production and Partial Purification of Laccase from Ash Gourd Peels
Surabhi Mitra1,2, Kanhaiya Tiwari1 and Shailendra Kumar Srivastava1*
1Department of Biochemistry and Biochemical Engineering, Jacob School of Biotechnology and Bioengineering, Sam Higginbottom Institute of Agriculture Technology and Sciences, Allahabad-211007, India
2Department of Biotechnology, MNNIT, Allahabad- 211004, India
*Corresponding author
Abstract:

The aim of the present study was to optimize a process for production enzyme Laccase via microbial fermentation using peels of Ash Gourd. It is rich in various nutritional substances which are required for culture of microorganisms.. By estimating proximate values of various substances a synthetic media was prepared for Trichoderma Viride. Laccases were collected extra and intracellular level. The enzyme activity, growth was observed which were treated in various conditions and purified via various steps i.e. Ammonium Sulphate and dialysis. After dialysis activity in different conditions were 0.1N HCl- 30.17, 0.1N NaOH-23.17, Boiled- 22.05, Experimental Noramal- 22.05, and Sonicated- 37.09.


Keywords: Laccase,Ash Gourd, Trichoderma viride
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How to cite this article:

Surabhi Mitra, Kanhaiya Tiwari, Shailendra Kumar Srivastava. 2017. Optimization for Production and Partial Purification of Laccase from Ash Gourd Peels.Int.J.Curr.Microbiol.App.Sci. 6(2): 997-1003. doi: http://dx.doi.org/10.20546/ijcmas.2017.602.112