National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Antibiotics are the first line of defence against bacterial infections. Overuse/misuse of antibiotics has led to the emergence of antibiotics resistant bacterial strains, making them ineffective. Alternative approaches should be sought to handle this challenge. Bile salt is a cation compounded form of bile acid and is synthesized in the liver. Reduction in the flow of bile salts has led to bacterial translocation in the body. Therefore, the aim of the present study was to evaluate the in vitro effect of bile salt on E. coli and S. aureus. This work also investigated the additive effect of bile salt in combination with other antibiotics namely gentamycin and ciprofloxacin. Bile salt exhibited maximum zone of inhibition (ZI) of 4.8±0.28mm against E. coli while against S. aureus, 12.0±0mm ZI was observed. While exploring the additive effect of bile salt, it enhanced the ZI of gentamycin and ciprofloxacin against both the microorganisms. Bile salt was also found effective in damaging the cell membrane of S. aureus which was evident by nucleic acid and protein leakage. This effect remained absent with E. coli. Thus, bile salt showed significant antibacterial activity against gram-positive microorganism while gram-negative remained resistant. Bile salt also exhibited additive effect in combination with gentamycin and ciprofloxacin.
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