Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2019) [Effective from January 1, 2019]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(10): 2430-2436
DOI: https://doi.org/10.20546/ijcmas.2019.810.282


Evaluation of Antibacterial Effects of Bile Salt on Pathogenic Bacteria- An in vitro Study
Anuradha Tyagi*, Vanita Gupta and Aseem Bhatnagar
Department of Capacity Enhancement & Product Implementation, Institute of Nuclear Medicine and Allied sciences, Brig S. K. Mazumdar Road, Delhi- 110054, India
*Corresponding author
Abstract:

Antibiotics are the first line of defence against bacterial infections. Overuse/misuse of antibiotics has led to the emergence of antibiotics resistant bacterial strains, making them ineffective. Alternative approaches should be sought to handle this challenge. Bile salt is a cation compounded form of bile acid and is synthesized in the liver. Reduction in the flow of bile salts has led to bacterial translocation in the body. Therefore, the aim of the present study was to evaluate the in vitro effect of bile salt on E. coli and S. aureus. This work also investigated the additive effect of bile salt in combination with other antibiotics namely gentamycin and ciprofloxacin. Bile salt exhibited maximum zone of inhibition (ZI) of 4.8±0.28mm against E. coli while against S. aureus, 12.0±0mm ZI was observed. While exploring the additive effect of bile salt, it enhanced the ZI of gentamycin and ciprofloxacin against both the microorganisms. Bile salt was also found effective in damaging the cell membrane of S. aureus which was evident by nucleic acid and protein leakage. This effect remained absent with E. coli. Thus, bile salt showed significant antibacterial activity against gram-positive microorganism while gram-negative remained resistant. Bile salt also exhibited additive effect in combination with gentamycin and ciprofloxacin.


Keywords: Bile salt, E. coli, S. aureus, Antimicrobial
Download this article as Download

How to cite this article:

Anuradha Tyagi, Vanita Gupta and Aseem Bhatnagar. 2019. Evaluation of Antibacterial Effects of Bile Salt on Pathogenic Bacteria- An in vitro Study.Int.J.Curr.Microbiol.App.Sci. 8(10): 2430-2436. doi: https://doi.org/10.20546/ijcmas.2019.810.282