International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
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Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(10): 2282-2295

In vitro Propagation of Citrus Species through Callus Induction and Regeneration: A Review
Mudasir Iqbal1*, V. K. Wali1, Parshant Bakshi1, Kiran Kour1, Vijay K. Razdan2, B. K. Sinha2 and K. K. Sood3
1Division of Fruit Science,
2Division of Plant Pathology,
Division of Agroforestry, Faculty of Agriculture, Main Campus, Chatha, Sher-e-Kashmir University of Agricultural Sciences & Technology of Jammu, J&K-180009, India
*Corresponding author

Citrus, one of the most important group of fruit crops around the world, are propagated at large scale with many difficulties. Propagation through seeds is challenging because of Phytophthora foot rot together with recalcitrance of citrus seeds. Vegetative propagation of Citrus species is mainly performed now-a-days by budding on seedling rootstocks. As heavy losses are experienced among the susceptible seedlings due to Phytophthora and Citrus tristeza virus (CTV), the interest in resistant rootstocks has greatly increased. The potential of conventional methods of citrus plant breeding of rootstocks are limited by physiological factors such as heterozygosity, inbreeding depression, nucellar polyembryony and juvenility. Under such conditions advanced tissue culture techniques provide best possible alternative for producing large number of resistant progenies from elite citrus genotypes. Plant tissue culture provides reliable and economical method of maintaining pathogen free plants that allows rapid multiplication and international exchange of germplasm. Generally, when in vitro propagation protocols are developed for any specific plant species, specialized conditions for individual genotypes, elite species and even various developmental stages of the explants plants are selected via error-and- trial experiments. Because large diversity is observed in Citrus plant family, it takes many months to develop protocols for most suitable culture medium, best concentrations and combinations of plant growth regulators and other supplements for better development of explant cultures. Therefore, in this review, we tried to put together results from difficult-to-find literatures and listed all the identified findings, in which callus induction or somatic organogenesis was used to develop citrus plants. Successful protocols of surface sterilization method, culture establishment, shoot regeneration, in vitro rooting and acclimatization are presented systematically.

Keywords: Tissue culture, Micropropagation, Callus, Regeneration, Rooting, Acclimatization
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How to cite this article:

Mudasir Iqbal, V. K. Wali, Parshant Bakshi, Kiran Kour, Vijay K. Razdan, B. K. Sinha and Sood, K. K. 2019. In vitro Propagation of Citrus Species through Callus Induction and Regeneration: A Review.Int.J.Curr.Microbiol.App.Sci. 8(10): 2282-2295. doi: