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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(10): 2145-2150
DOI: https://doi.org/10.20546/ijcmas.2019.810.249


Expression of Bovine Viral Diarrhoea Virus E2 Gene in HeLa Cell Line
Nitin Sharma* and Mahesh Kumar
Division of Animal Cell Culture Laboratory, Institute of Biotechnology, G.B. Pant University of Agriculture & Technology, Patwadangar (Nainital)-263128, UK, India
*Corresponding author
Abstract:

Bovine viral diarrhoea virus (BVDV) is the most prevalent infectious disease of cattle. The envelope glycoprotein (E2) gene of an Indian BVDV isolate was already cloned. In this study, recombinant plasmid pcDNA.BVDV.E2 was used to transfect 70-80% HeLa cell, expressed E2 protein which was confirmed by immunoperoxidase test. Rabit anti mouse HRPO conjugate was used in expression analysis and anti BVDV-E2 hyperimmune serum was raised in mice using recombinant plasmid pcDNA.BVDV.E2 for develop specific antibody. This study demonstrates feasibility of BVDV E2 protein expression in HeLa cell and its efficacy as an antigen in ELISA for detecting BVDV neutralizing antibodies in cattle.


Keywords: BVDV, E2, Expression, Eukaryotic system
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How to cite this article:

Nitin Sharma and Mahesh Kumar 2019. Expression of Bovine Viral Diarrhoea Virus E2 Gene in HeLa Cell Line.Int.J.Curr.Microbiol.App.Sci. 8(10): 2145-2150. doi: https://doi.org/10.20546/ijcmas.2019.810.249