National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Bovine viral diarrhoea virus (BVDV) is the most prevalent infectious disease of cattle. The envelope glycoprotein (E2) gene of an Indian BVDV isolate was already cloned. In this study, recombinant plasmid pcDNA.BVDV.E2 was used to transfect 70-80% HeLa cell, expressed E2 protein which was confirmed by immunoperoxidase test. Rabit anti mouse HRPO conjugate was used in expression analysis and anti BVDV-E2 hyperimmune serum was raised in mice using recombinant plasmid pcDNA.BVDV.E2 for develop specific antibody. This study demonstrates feasibility of BVDV E2 protein expression in HeLa cell and its efficacy as an antigen in ELISA for detecting BVDV neutralizing antibodies in cattle.
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