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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(10): 1415-1420
DOI: https://doi.org/10.20546/ijcmas.2019.810.166


Genetic Polymorphism of MHC Class II DRB3 Gene in Salem Black Goats by PCR- RFLP
S. B. Thirunavukkarasu1*, Pushpendra Kumar1, Om Prakash1, Amit Kumar1, Nihar Ranjan Sahoo1, G. V. P. P. S. Ravikumar1, Bharat Bhushan1, K. Ilayakumar1, V. Sharavanan2 and N. Nagarajan3
1Molecular Genetics Lab, Division of Animal Genetics, ICAR-Indian Veterinary Research Institute (IVRI), Izatnagar, Bareilly, Uttar Pradesh- 243122, India
2Director of Animal Husbandry and Veterinary Services, Chennai- 600 006, India
3Veterinary Assistant Surgeon, Sheep Farm, Chinnasalem, Villupuram- 606 201, India
*Corresponding author
Abstract:

The highly polymorphic gene of the MHC is central molecule in immune system in recognition of pathogens and parasites. The purpose of this study was to study the polymorphism of CLA-DRB3 gene in Salem black goat using PCR-RFLP technique. A region of exon 2 encompassing 285 bp fragment of DRB 3 gene was amplified by polymerase chain reaction. The restriction digestion by TaqI revealed three genotypes AA (285 bp), AB (285/163/122 bp) and BB (163/122 bp) with frequencies 0.220, 0.490 and 0.290, respectively and two alleles A and B with frequencies 0.465 and 0.535, respectively. The polymorphic information content (PIC) value and expected heterozygosity were 0.374 and 0.490, respectively which were low in both cases. The present study showed polymorphic nature of MHC Class II DRB3 Gene in Salem black goats at this locus and the frequencies of heterozygote were greater than homozygote.


Keywords: SNPs technique, PCR – RFLP, MHC class II DRB3, TaqI
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How to cite this article:

Thirunavukkarasu, S. B., Pushpendra Kumar, Om Prakash, Amit Kumar, Nihar Ranjan Sahoo, G. V. P. P. S. Ravikumar, Bharat Bhushan, K. Ilayakumar, V. Sharavanan and Nagarajan, N. 2019. Genetic Polymorphism of MHC Class II DRB3 Gene in Salem Black Goats by PCR- RFLP.Int.J.Curr.Microbiol.App.Sci. 8(10): 1415-1420. doi: https://doi.org/10.20546/ijcmas.2019.810.166