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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(10): 1386-1393
DOI: https://doi.org/10.20546/ijcmas.2019.810.162


Studies on Effect of Hormone on in vitro Regeneration of Tinospora cordifolia
Anika Malik* and Aditi Arya
Department of Biotechnology (Plant Tissue Culture and Genetic Engineering Lab), Deenbandhu Chhoturam University of Science and Technology, Murthal, Sonipat, Haryana, India, 131001
*Corresponding author
Abstract:

Research in plant biotechnology is playing a crucial role in the production and conservation of plant-based resources globally. The aim of the work was to establish favourable culture medium condition for regeneration and better growth of Tinospora cordifolia explant. Plant Micropropagation has also been used as a tool for the propagation of genetically manipulated superior clones. Our attempt was made to develop micropropagation as suitable condition for cloning of Tinospora cordifolia. The explants were cut into desirable size and inoculated into regeneration medium consisting of Murashige and Skoog basal combination and various combinations of hormones. The cultures were incubated at 16:8 hrs photoperiod and temperature of 27 + 2°. Nodal explants produced proliferation of shoots on the medium containing nodal explants produced proliferation of shoots on the medium containing BAP and IAA at different combinations. Results were obtained showing regeneration frequency after 14 and 21 days.


Keywords: Tinospora cordifolia, Micropropagation, Explants, BAP, IAA
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How to cite this article:

Anika Malik and Aditi Arya. 2019. Studies on Effect of Hormone on in vitro Regeneration of Tinospora cordifolia.Int.J.Curr.Microbiol.App.Sci. 8(10): 1386-1393. doi: https://doi.org/10.20546/ijcmas.2019.810.162