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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
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Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(10): 1338-1346
DOI: https://doi.org/10.20546/ijcmas.2019.810.156


Prevalence and Drug Resistance Pattern of Escherichia coli Strains Isolated from Milk and Milk Products
Monika Soni1*, Abhishek Gaurav1, Bincy Joseph2, S. S. Shekhawat and Subhash Chand Meena1
1Department of Veterinary Public Health, College of Veterinary and Animal Science, Navania, Udaipur, India
2Department of Veterinary Microbiology, College of Veterinary and Animal Science, Navania, Udaipur, India
*Corresponding author
Abstract:

In the present study prevalence and antibiotic susceptibility pattern of Escherichia coli isolated from milk and milk products from retail dairies of different parts of Udaipur city, Rajasthan were determined. The phenotypic and genotypic characterization of E. coli isolates was done to determine its prevalence and antibiotic susceptibility pattern. A total of 150 samples comprising of raw pooled milk (n=30), pasteurized milk (n=30), dahi (n=30), paneer (n=30), and sweets (burfi) (n=30) were processed for the isolation of E. coli. Out of 150 samples, the prevalence of E. coli was recorded in raw pooled milk, dahi, paneer, pasteurised milk and sweets (burfi) samples as 76.66% (23), 33.33% (10), 20% (6), 0% (0) and 43.33% (13), respectively. The analysis of antibiogram revealed that the most effective antibiotic was Chloramphenicol (91.30%), followed by Trimethoprim to which 86.95% of the isolates were sensitive. Also, 82.60% isolates were sensitive to Gentamicin and Ciprofloxacin, 78.26% to Ceftriaxone, 73.91% to Co-Trimoxazole and other antibiotics were still less effective. Penicillin-G showed highest resistance (100.00%) followed by Methicillin (91.30%), while 52.17% isolates were resistant to Ampicillin, 43.47% to Erythromycin and Carbenicillin, 21.73% to Tetracycline, 17.39% to Ceftriaxone and other antibiotics were still less resistant. Out of 50 E. coli isolates from milk and milk products, only 11 isolates (22%) were found to be positive for blaCTX-M gene while only 2 isolates (1.25%) were found to be positive for stx1 gene. These results indicate that the milk and milk products sold in the study area have high level of antibiotic resistant E coli which is a public health concern. Therefore, stringent hygienic measures and prudent use of antibiotics should be practiced to improve the present worrisome situation.


Keywords: Escherichia coli, Antibiotic resistance, Virulence, Prevalence, Milk
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How to cite this article:

Monika Soni, Abhishek Gaurav, Bincy Joseph, S. S. Shekhawat and Subhash Chand Meena. 2019. Prevalence and Drug Resistance Pattern of Escherichia coli Strains Isolated from Milk and Milk Products.Int.J.Curr.Microbiol.App.Sci. 8(10): 1338-1346. doi: https://doi.org/10.20546/ijcmas.2019.810.156