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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(10): 1290-1308
DOI: https://doi.org/10.20546/ijcmas.2019.810.152


Synergistic Effect of PPFD and Mycorrhization for Efficient in vitro Propagation of Dendrobium chrysanthum Wall. ex Lindl.
Subarna Hajong1*, Suman Kumaria2 and Pramod Tandon3
1ICAR-National Bureau of Plant Genetic Resources Regional Station Shillong, Umiam, Meghalaya 793103, India
2Plant Biotechnology Laboratory, Department of Botany, North-Eastern Hill University, Shillong 793022, India
3Biotech Park, Lucknow, Uttar Pradesh 226021, India
*Corresponding author
Abstract:

Integration of optimal photosynthetic photon flux density (PPFD) and mycorrhization under in vitro conditions appears to have significant synergistic effect in enhancing the growth and survival of the medicinal orchid, Dendrobium chrysanthum under in vitro and ex vitro conditions. PPFD at 60 µmol m-2s-1 was found to be optimal for root colonization for mycorrhizal association by fungi (73% colonization). The growth of mycorrhizal plantlets was observed to be superior at 60 µmol m-2s-1 PPFD as compared to plantlets subjected to either lower or higher PPFD. The growth responses in terms of shoot length, leaf area, fresh weight (FW) and dry weight (DW) of mycorrhizal plantlets were consistently and significantly higher at 60 µmol m-2s-1 PPFD after 30, 60 and 90 days of treatment. PPFD affected development of stomata with increase in number of stomata per unit area of leaf with increasing light intensity. Maximum length of stomata was observed in mycorrhizal plantlets at 60 µmol m-2s-1 PPFD but light intensity did not cause any substantial effect on width of stomata. After 120 days of hardening, highest survival percentage (97.33%) was obtained with mycorrhizal plantlets transferred from 60 µmol m-2s-1 PPFD. Growth parameters observed were also higher in mycorrhizal plantlets transferred from 60 µmol m-2s-1 PPFD. Mycorrhizal association in vitro at optimal PPFD produced the most marked growth response both under in vitro conditions and during ex vitro acclimatization.


Keywords: Dendrobium chrysanthum, Ex vitro, In vitro Mycorrhization, PPFD
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How to cite this article:

Subarna Hajong, Suman Kumaria and Pramod Tandon. 2019. Synergistic Effect of PPFD and Mycorrhization for Efficient in vitro Propagation of Dendrobium chrysanthum Wall. ex Lindl..Int.J.Curr.Microbiol.App.Sci. 8(10): 1290-1308. doi: https://doi.org/10.20546/ijcmas.2019.810.152